WB, FC-FP
H
Endogenous
75
Rabbit IgG
#Q13641
7162
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Flow Cytometry (Fixed/Permeabilized) | 1:800 - 1:3200 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His140 of human TPBG/5T4 protein.
Background
Trophoblast glycoprotein (TPBG/5T4) is a type I transmembrane glycoprotein that is normally expressed by trophoblast cells of the placenta with a very limited expression pattern in normal adult tissues (1,2). The extracellular domain of TPBG is extensively glycosylated and contains multiple leucine-rich repeats while its cytoplasmic domain consists of a PDZ domain-binding motif, which is important for linking TPBG to intracellular signaling networks involved in the regulation of cell motility and adhesion (3,4). Research studies have shown that cell surface expression of TPBG plays a critical role in modulating signaling cascades that drive cell adhesion, morphology, and motility processes that are fundamental for normal progression of embryogenesis (5-7).Research studies have demonstrated that TPBG is aberrantly overexpressed in numerous types of solid tumors (8) and functions to promote enhanced tumor cell motility and metastasis (9,10). In some tumors, such as NSCLC and HNSCC, TPBG has been identified as a novel marker of tumor-initiating cells (11,12). The observed differential expression of TPBG by normal tissue versus tumor tissue has been exploited by multiple immunotherapeutic agents that are currently being evaluated for targeting of multiple types of solid tumors (13).
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- Woods, A.M. et al. (2002) Biochem J 366, 353-65.
- Shaw, D.M. et al. (2002) Biochem J 363, 137-45.
- Awan, A. et al. (2002) Biochem Biophys Res Commun 290, 1030-6.
- Barrow, K.M. et al. (2005) Dev Dyn 233, 1535-45.
- Ward, C.M. et al. (2003) J Cell Sci 116, 4533-42.
- Ward, C.M. et al. (2006) Exp Cell Res 312, 1713-26.
- Southall, P.J. et al. (1990) Br J Cancer 61, 89-95.
- Carsberg, C.J. et al. (1996) Int J Cancer 68, 84-92.
- Myers, K.A. et al. (1994) J Biol Chem 269, 9319-24.
- Damelin, M. et al. (2011) Cancer Res 71, 4236-46.
- Kerk, S.A. et al. (2017) Clin Cancer Res 23, 2516-2527.
- Stern, P.L. and Harrop, R. (2017) Cancer Immunol Immunother 66, 415-426.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting FC-FP: Flow Cytometry (Fixed/Permeabilized)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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