WB, IF-F
H M R
Endogenous
52
Rabbit IgG
#Q8IWU9
121278
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Frozen) | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu30 of human TPH2 protein.
Background
Tryptophan hydroxylase (TPH) is the rate-limiting enzyme in the biosynthesis of serotonin (1) by converting tryptophan to 5-hydroxy-L-tryptophan (2). Two isoforms of TPH exist: TPH-1 is mainly expressed in the periphery, whereas the expression of TPH-2 is restricted to neuronal cells and the central nervous system (3). Most of the serotonin found throughout the body is synthesized by TPH-1 in enterochromaffin cells of the gastrointestinal tract. Targeted disruption of the tph1 gene results in low levels of circulating and tissue serotonin (4).
Polymorphisms and regulation of the tph2 gene are involved in cognitive, emotional, and psychiatric disorders (5-7)
- Hale, M.W. et al. (2011) J Chem Neuroanat 41, 219-26.
- Silva, S.R. et al. (2011) Anticancer Res 31, 1153-60.
- Walther, D.J. et al. (2003) Science 299, 76.
- Amireault, P. et al. (2011) Proc Natl Acad Sci U S A 108, 13141-6.
- Waider, J. et al. (2011) Psychoneuroendocrinology 36, 393-405.
- Choong, M.Y. et al. (2014) Psychiatry Res 220, 1163-6.
- Zhang, Y. et al. (2015) Mol Med Rep 12, 3184-90.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IF-F: Immunofluorescence (Frozen)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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