WB, IF-F, IF-IC
H M R
Endogenous
23
Rabbit
#Q01995
6876
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunofluorescence (Frozen) | 1:100 - 1:200 |
Immunofluorescence (Immunocytochemistry) | 1:100 - 1:200 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly171 of human Transgelin/TAGLN protein. Antibodies are purified by peptide affinity chromatography.
Background
Transgelin (TAGLN, SM22α) is an actin-binding protein of the calponin family. It contains an amino-terminal calponin homology domain and a carboxy-terminal calponin-like module domain required for actin binding (1,2). TAGLN is a TGF-β inducible gene that is abundantly expressed in visceral and vascular smooth muscle cells, and is often used as an early marker of smooth muscle differentiation (3-5). TAGLN expression has also been shown in brain pericytes, providing use in studying the blood brain barrier (6). Altered levels of TAGLN are seen in multiple cancer types, including colorectal, breast, and prostate cancer (4).
- Assinder, S.J. et al. (2009) Int J Biochem Cell Biol 41, 482-6.
- Fu, Y. et al. (2000) J Appl Physiol (1985) 89, 1985-90.
- Hirschi, K.K. et al. (1998) J Cell Biol 141, 805-14.
- Dvorakova, M. et al. (2014) Expert Rev Proteomics 11, 149-65.
- Huang, L. et al. (2018) J Cell Mol Med 22, 6249-6261.
- Blanchard, J.W. et al. (2020) Nat Med 26, 952-963.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IF-F: Immunofluorescence (Frozen) IF-IC: Immunofluorescence (Immunocytochemistry)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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