WB
H M R Mk
Endogenous
68
Rabbit IgG
#P29401
7086
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val296 of human transketolase protein.
Background
Transketolase (TKT) is a homodimer in the pentose phosphate pathway (PPP) that catalyzes the interketol transfer between ketoses and aldoses (1,2). This enzyme, along with transaldolase, connects the nonoxidative branch of the PPP with glycolysis (1-3). Several regions of TKT are evolutionarily conserved from gram-negative bacteria to mammals (3). There is evidence that hypoxic (4) and non-hypoxic induction of HIF1-α (5) increases the expression of TKT. Because cancer cells rely on TKT in altered cell metabolism for nucleic acid synthesis, work has been done to develop inhibitors of TKT as novel cancer treatments (5-8).
- Mitschke, L. et al. (2010) J Biol Chem 285, 31559-70.
- Esakova, O.A. et al. (2005) Life Sci 78, 8-13.
- Schenk, G. et al. (1997) J Mol Evol 44, 552-72.
- Haseloff, R.F. et al. (2006) Proteomics 6, 1803-9.
- Zhao, F. et al. (2010) Oncogene 29, 2962-72.
- Thomas, A.A. et al. (2008) Bioorg Med Chem Lett 18, 2206-10.
- Thomas, A.A. et al. (2008) Bioorg Med Chem Lett 18, 509-12.
- Raïs, B. et al. (1999) FEBS Lett 456, 113-8.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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