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55016
Traumatic Brain Injury Biomarker Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

Traumatic Brain Injury Biomarker Antibody Sampler Kit #55016

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Simple Western™ analysis of lysates (0.1 mg/mL) from mouse brain using PSD95 (D27E11) XP® Rabbit mAb #3450. The virtual lane view (left) shows the target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 66-440 kDa separation module.
Simple Western analysis of lysates (0.1 mg/mL) from Mouse Brain Tissue Extracts using Tau (D1M9X) XP® Rabbit #46687. The virtual lane view (left) shows the target band (as indicated) and a band corresponding to Tau (as indicated) at 1:50 and 1:250 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:50 (blue line) and 1:250 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.
Confocal immunofluorescent analysis of fixed frozen mouse cerebellum using Tau (D1M9X) XP® Rabbit mAb (green), TMEM119 (E4B9S) Mouse mAb #98778 (red) and ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Confocal immunofluorescent analysis of fixed frozen mouse hippocampus using Tau (D1M9X) XP® Rabbit mAb (green), TMEM119 (E4B9S) Mouse mAb #98778 (red) and ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Immunohistochemical analysis of paraffin-embedded normal cynomolgus monkey brain using GFAP (E4L7M) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded normal cynomolgus monkey brain using GFAP (E4L7M) XP® Rabbit mAb.
Confocal immunofluorescent analysis of U-251 MG cells (left, positive) or HeLa cells (right, negative) using GFAP (E4L7M) XP® Rabbit mAb #80788 (green). Actin filaments were labeled with DyLight 650 Phalloidin #12956 (red). Blue = DAPI #4083 (fluorescent DNA dye).
Western blot analysis of extracts from various cell lines using UCHL1 (D3T2E) XP® Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).
Western blot analysis of extracts from mouse brain, HeLa cells and rat brain, using Neurofilament-L (C28E10) Rabbit mAb.
Western blot analysis of extracts from human cerebellum and rat brain using PSD95 (D27E11) XP® Rabbit mAb.
Western blot analysis of normal mouse brain and Tau KO (-/-) mouse brain with Tau (D1M9X) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). Tau-KO mouse brain tissue was kindly provided by Dr. Dominic Walsh at Brigham and Women's Hospital and Harvard Medical School.
Western blot analysis of extracts from mouse brain, rat brain, human brain, and mouse liver using Enolase-2 (E7D7I) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
Western blot analysis of extracts from mouse brain and C2C12 cells using Myelin Basic Protein (D8X4Q) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western blot analysis of extracts from various tissues and cell lines using GFAP (E4L7M) XP® Rabbit mAb and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Immunohistochemical analysis of paraffin-embedded normal human brain using GFAP (E4L7M) XP® Rabbit mAb performed on the Leica BOND Rx.
Western blot analysis of extracts from various cell lines using S100B (E7C3A) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western blot analysis of extracts from various tissues using S100B (E7C3A) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Confocal immunofluorescent analysis of fixed frozen mouse spleen using S100B (E7C3A) Rabbit mAb (green). After blocking free secondary binding sites with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, sections were incubated with CD3 (17A2) Rat mAb (FITC Conjugate) #86603 (cyan pseudocolor), Iba1/AIF-1 (E4O4W) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) #78060 (red), and ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Confocal immunofluorescent analysis of fixed frozen mouse cerebral cortex using S100B (E7C3A) Rabbit mAb (red). After blocking free secondary binding sites with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, sections were incubated with HS1 (D5A9) XP® Rabbit mAb (Rodent Specific) (Alexa Fluor® 488 Conjugate) #68206 (green) and ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Confocal immunofluorescent analysis of fixed frozen mouse hippocampus using S100B (E7C3A) Rabbit mAb (red). After blocking free secondary binding sites with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, sections were incubated with HS1 (D5A9) XP® Rabbit mAb (Rodent Specific) (Alexa Fluor® 488 Conjugate) #68206 (green), NeuN (D4G4O) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) #62994 (cyan pseudocolor), and ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Confocal immunofluorescent analysis of SK-MEL-28 cells (left, positive) or T-47D cells (right, negative) using S100B (E7C3A) Rabbit mAb (green), DyLight 554 Phalloidin #13054 (red), and DAPI #4083 (blue).
Immunohistochemical analysis of paraffin-embedded human soft tissue squamous cell carcinoma using S100B (E7C3A) Rabbit mAb.
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing Myc/DDK-tagged full-length human UCHL1 (hUCHL1-Myc/DDK; +), Myc/DDK-tagged full-length human UCHL3 (hUCHL3-Myc/DDK; +), Myc/DDK-tagged full-length human UCHL5 (hUCHL5-Myc/DDK; +), and Myc/DDK-tagged full-length human BAP1 (hBAP1-Myc/DDK; +), using UCHL1 (D3T2E) XP® Rabbit mAb (upper) or DYKDDDDK Tag Antibody #2368 (lower).
Immunohistochemical analysis of paraffin-embedded mouse brain using Neurofilament-L (C28E10) Rabbit mAb.
Confocal immunofluorescent analysis of rat cerebellum and retina using PSD95 (D27E11) XP® Rabbit mAb (red) and Neurofilament-L (DA2) Mouse mAb #2835 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Western blot analysis of extracts from various cell lines and tissues using Tau (D1M9X) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Confocal immunofluorescent analysis of mouse tissue showing neural-specific staining of the cortex (left-top), spinal cord (right-top), enteric nerves of the colon (left-bottom), and nerve fibers of renal arterioles (right-bottom), using Enolase-2 (E7D7I) Rabbit mAb (green). Sections are mounted with ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Immunohistochemical analysis of a paraffin-embedded human Alzheimer's brain using Myelin Basic Protein (D8X4Q) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human medulloblastoma using GFAP (E4L7M) XP® Rabbit mAb performed on the Leica BOND Rx.
Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using S100B (E7C3A) Rabbit mAb.
Western blot analysis of extracts from Ramos and K-562 cells using UCHL1 (D3T2E) XP® Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). As expected, UCHL1 protein expression is not detected in K-562 cells.
Immunohistochemical analysis of paraffin-embedded human brain using Neurofilament-L (C28E10) Rabbit mAb in the presence of control peptide (left) or Neurofilament-L blocking peptide #1005 (right).
Immunohistochemical analysis of paraffin-embedded human Alzheimer's brain using Tau (D1M9X) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
Confocal immunofluorescent analysis of TT cells (left, positive) or Huh7 cells (right, negative) using Enolase-2 (E7D7I) Rabbit mAb (green). Nuclei were labeled with DAPI #4083 (blue).
Immunohistochemical analysis of a paraffin-embedded mouse brain using Myelin Basic Protein (D8X4Q) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using S100B (E7C3A) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using UCHL1 (D3T2E) XP® Rabbit mAb.
Confocal immunofluorescent analysis of normal rat cerebellum using Neurofilament-L (C28E10) Rabbit mAb (green) and GFAP (GA5) Mouse mAb #3670 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Immunohistochemical analysis of paraffin-embedded human normal appendix using Tau (D1M9X) XP® Rabbit mAb.
Flow cytometric analysis of THP-1 cells (blue) and TT cells (green) using Enolase-2 (E7D7I) Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Confocal immunofluorescent analysis of rat brain using Myelin Basic Protein (D8X4Q) XP® Rabbit mAb (green) and Synaptophysin (7H12) Mouse mAb (IF Formulated) #9020 (red). Blue pseudocolor = DRAQ5®#4084 (fluorescent DNA dye).
Immunohistochemical analysis of paraffin-embedded human glioblastoma using GFAP (E4L7M) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human ductal breast carcinoma using S100B (E7C3A) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded DU 145 (left; positive) and LNCaP (right; negative) cell pellets using UCHL1 (D3T2E) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded T-47D cell pellet (left, positive) or MDA-MB-231 cell pellet (right, negative) using Tau (D1M9X) XP® Rabbit mAb.
Confocal immunofluorescent analysis of mouse primary neurons and neuroglia using Myelin Basic Protein (D8X4Q) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Immunohistochemical analysis of paraffin-embedded human medulloblastoma using GFAP (E4L7M) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human granulosa cell tumor of the ovary (left), prostate adenocarcinoma (middle), or ovarian serous carcinoma (right) using S100B (E7C3A) Rabbit mAb (top) or S100B Rabbit mAb (bottom). These two antibodies detect unique, non-overlapping epitopes on S100B protein. The similar staining patterns obtained with both antibodies help to confirm the specificity of the staining.
Immunohistochemical analysis of paraffin-embedded colorectal adenocarcinoma using UCHL1 (D3T2E) XP® Rabbit mAb in the presence of control peptide (left) or antigen specific peptide (right).
Immunohistochemical analysis of paraffin-embedded mouse lung using Tau (D1M9X) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse hippocampus using GFAP (E4L7M) XP® Rabbit mAb (left) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
Immunohistochemical analysis of paraffin-embedded normal human pancreas using S100B (E7C3A) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human prostate adenocarcinoma using UCHL1 (D3T2E) XP® Rabbit mAb.
Confocal immunofluorescent analysis of fixed frozen mouse striatum using Tau (D1M9X) XP® Rabbit mAb (green), TMEM119 (E4B9S) Mouse mAb #98778 (red) and ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Immunohistochemical analysis of various paraffin-embedded normal mouse tissues: brain cortex (top-left), colon (top-center), small intestine (top-right), liver (bottom-left), prostate (bottom-center) and skeletal muscle (bottom-right) using GFAP (E4L7M) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded rat brain, cortex (left) and cerebellum (right), using GFAP (E4L7M) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded normal human thymus using S100B (E7C3A) Rabbit mAb.
Confocal immunofluorescent analysis of mouse brain using UCHL1 (D3T2E) XP® Rabbit mAb (green). Blue = Hoescht 33342 #4082 (fluorescent DNA dye).
Confocal immunofluorescent analysis of T-47D (positive, left) or MDA-MB-231 (negative, right) cells using Tau (D1M9X) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Confocal immunofluorescent analysis of adult mouse cerebellum (left) and hippocampus (right) using GFAP (E4L7M) XP® Rabbit mAb (green). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Immunohistochemical analysis of paraffin-embedded normal human spleen using S100B (E7C3A) Rabbit mAb.
Confocal immunofluorescent analysis of mouse brain using UCHL1 (D8R2I) XP® Rabbit mAb (green). Blue = Hoescht 33342 #4082 (fluorescent DNA dye).
Immunohistochemical analysis of paraffin-embedded human squamous cell carcinoma of the tonsil using S100B (E7C3A) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
Confocal immunofluorescent analysis of mouse olfactory bulb (left) and pons (right) using UCHL1 (D3T2E) XP® Rabbit mAb #13179 (green) and GFAP (GA5) Mouse mAb (Alexa Fluor® 555 Conjugate) #3656 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Immunohistochemical analysis of paraffin-embedded SK-MEL-28 cell pellet (left, positive) or T-47D cell pellet (right, negative) using S100B (E7C3A) Rabbit mAb.
Confocal immunofluorescent analysis of DU 145 (positive; left) and LNCaP (negative; right) cells using UCHL1 (D3T2E) XP® Rabbit mAb (green). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye).
Flow cytometric analysis of K-562 cells (blue; negative) and Ramos cells (green; positive) using UCHL1 (D3T2E) XP® Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
To Purchase # 55016
Cat. # Size Qty. Price
55016T
1 Kit  (8 x 20 microliters)

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
GFAP (E4L7M) XP® Rabbit mAb 80788 20 µl
  • WB
  • IHC
  • IF
H M R Mk 50 Rabbit IgG
S100B (E7C3A) Rabbit mAb 90393 20 µl
  • WB
  • IHC
  • IF
H M R 10 Rabbit IgG
UCHL1 (D3T2E) XP® Rabbit mAb 13179 20 µl
  • WB
  • IHC
  • IF
  • F
H M R Mk 27 Rabbit IgG
Neurofilament-L (C28E10) Rabbit mAb 2837 20 µl
  • WB
  • IHC
  • IF
H M R 70 Rabbit IgG
Enolase-2 (E7D7I) Rabbit mAb 65162 20 µl
  • WB
  • IF
  • F
H M R 47 Rabbit IgG
Tau (D1M9X) XP® Rabbit mAb 46687 20 µl
  • WB
  • IHC
  • IF
H M R 50-80 Rabbit IgG
Myelin Basic Protein (D8X4Q) XP® Rabbit mAb 78896 20 µl
  • WB
  • IHC
  • IF
H M R 12-18 Rabbit IgG
PSD95 (D27E11) XP® Rabbit mAb 3450 20 µl
  • WB
  • IF
H M R 95 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

Product Description

The Traumatic Brain Injury Biomarker Antibody Sampler Kit provides an economical means of detecting proteins involved in traumatic brain injury. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.

Specificity / Sensitivity

Each antibody in the Traumatic Brain Injury Biomarker Antibody Sampler Kit detects endogenous levels of its target protein. S100B (E7C3A) Rabbit mAb recognizes human S100B protein and is also reactive with mouse S100B; however, this antibody is not suggested for immunohistochemical analysis of mouse tissues. UCHL1 (D3T2E) XP® Rabbit mAb does not cross-react with other UCH family members. Enolase-2 (E7D7I) Rabbit mAb does not cross-react with human Enolase-1 protein.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His86 of human S100B protein, Glu450 of human Neurofilament-L protein, Asp430 of human tau protein, Ala185 of human myelin basic protein, Gln53 of human PSD95 protein, residues near the carboxy terminus of human Enolase-2 protein and human UCHL1 protein, and recombinant protein specific to human GFAP protein.

Background

Traumatic brain injury (TBI) is a worldwide health issue that significantly affects the patient as well as their family. Annual total cost of nonfatal TBI in 2016 was $40.6 billion in the United States (1). In addition to acute brain injury, even mild cases, can lead to cognitive impairment and long-term psychiatric changes. More long term, TBI patients exhibit lower resilience to neurodegenerative disease-associated pathology (2). Treatment of TBI is made more difficult due to lack of reliable biomarkers to detect TBI (3). Several proteins are of interest, which are candidates for measurement in blood after TBI. Glial fibrillary acidic protein (GFAP) is an astrocytic intermediate filament protein. As a cytoskeletal protein, GFAP helps provide structural support to astrocytes, which provide metabolic support to neurons and maintains the blood brain barrier. The number and size of astrocytes, in a process called astrogliosis, is also positively correlated with brain injury (4). Also abundantly expressed in astrocytes, S100B is commonly used as an astrocytic marker and is positively correlated with TBI (5). Neurofilament-L (NfL) and tau are part of the neuronal cytoskeleton that provide structure to axons. Axons are covered by a multi-layered membrane called the myelin sheath. Myelin basic protein (MBP)  is enriched in myelin and helps maintain its structure. UCHL1 and Enolase-2 are ubiquitin hydrolases and glycolytic enzymes, respectively, that are enriched in neurons. PSD95 is an adaptor protein enriched at postsynaptic sites in neurons. After brain injury, neuron-enriched proteins, as well as proteins that maintain neuronal/axonal integrity, can be measured in the blood, reflecting neuronal damage (6). 

Pathways

Explore pathways related to this product.

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not for Use in Diagnostic Procedures.
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U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.
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