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61129
TRDC/TCRδ (E2E9T) XP® Rabbit mAb (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody

TRDC/TCRδ (E2E9T) XP® Rabbit mAb (BSA and Azide Free) #61129

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  1. WB
  2. IHC
Western Blotting Image 1: TRDC/TCRδ (E2E9T) XP® Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected (+) with constructs expressing Myc/DDK-tagged full-length human TRAC/TCRα constant protein (hTRAC/TCRα constant-Myc/DDK), Myc/DDK-tagged full-length human TRBC1/TCRβ constant region 1 protein (hTRBC1/TCRβ constant region 1-Myc/DDK), Myc/DDK-tagged full-length human TRBC2/TCRβ constant region 2 protein (hTRBC2/TCRβ constant region 2-Myc/DDK), Myc/DDK-tagged full-length human TRDC/TCRδ constant protein (hTRDC/TCRδ constant-Myc/DDK), and Myc/DDK-tagged full-length human TRGC1/TCRγ constant region 1 protein (hTRGC1/TCRγ constant region 1-Myc/DDK), using TRDC/TCRδ (E2E9T) XP® Rabbit mAb (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Data were generated using the standard formulation of this product.
Western Blotting Image 2: TRDC/TCRδ (E2E9T) XP® Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from PEER and Jurkat cell lines using TRDC/TCRδ (E2E9T) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: TRDC/TCRδ (E2E9T) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using TRDC/TCRδ (E2E9T) XP® Rabbit mAb performed on the Leica® BOND Rx. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: TRDC/TCRδ (E2E9T) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded normal human thymus using TRDC/TCRδ (E2E9T) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: TRDC/TCRδ (E2E9T) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded PEER cell pellet (left, positive) or Raji cell pellet (right, negative) using TRDC/TCRδ (E2E9T) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 3: TRDC/TCRδ (E2E9T) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human tonsil using TRDC/TCRδ (E2E9T) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 4: TRDC/TCRδ (E2E9T) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using TRDC/TCRδ (E2E9T) XP® Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 5: TRDC/TCRδ (E2E9T) XP® Rabbit mAb (BSA and Azide Free)

Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using TRDC/TCRδ (E2E9T) XP® Rabbit mAb. Data were generated using the standard formulation of this product.

Immunohistochemistry Image 6: TRDC/TCRδ (E2E9T) XP® Rabbit mAb (BSA and Azide Free)

Immunohistochemical analysis of paraffin-embedded human ductal breast carcinoma using TRDC/TCRδ (E2E9T) XP® Rabbit mAb. Data were generated using the standard formulation of this product.

Immunohistochemistry Image 7: TRDC/TCRδ (E2E9T) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded normal human spleen using TRDC/TCRδ (E2E9T) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 8: TRDC/TCRδ (E2E9T) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using TRDC/TCRδ (E2E9T) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 9: TRDC/TCRδ (E2E9T) XP® Rabbit mAb (BSA and Azide Free)

Immunohistochemical analysis of paraffin-embedded human T-cell lymphoma using TRDC/TCRδ (E2E9T) XP® Rabbit mAb. Data were generated using the standard formulation of this product.

To Purchase # 61129
Cat. # Size Qty. Price
61129SF
100 µg
$ 746

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 40-45
Source/Isotype Rabbit IgG

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • C&R-CUT&RUN
  • C&T-CUT&Tag
  • DB-Dot Blot
  • eCLIP-eCLIP
  • IF-Immunofluorescence
  • F-Flow Cytometry

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Usage Information

This product is the carrier-free version of product #55750. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free.

For standard formulation of this product see product #55750.

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

TRDC/TCRδ (E2E9T) XP® Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total TRDC/TCRδ protein. This antibody does not cross-react with human TRAC/TCRα constant, TRBC1/TCRβ constant region 1, TRBC2/TCRβ constant region 2, or TRGC1/TCRγ constant region 1.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with human TRDC/TCRδ recombinant protein.

Background

The γδ T Cell Receptor (TCR) is a heterodimer composed of a gamma chain and a delta chain expressed on the surface of T cells which participates in antigen recognition (1,2). Binding of gamma-delta TCR to antigen triggers phosphorylation of immunoreceptor tyrosine-based activation motifs (ITAMs) in CD3 chains of the antigen (3). Unlike αβ T cells, human γδ T cells recognize and kill transformed cells independently of human leukocyte antigen (HLA) restriction (3,4). γδ T lymphocytes develop largely in the thymus, generating their defining receptor via RAG-mediated rearrangement of a limited repertoire of variable (V), diversity (D), and joining (J) genes (3). In addition to the T cell receptor, γδ T cells usually also express activating natural killer (NK) receptors, which bind to stress-inducible surface molecules frequently expressed on malignant cells (4).
  1. Brenner, M.B. et al. Nature 322, 145-9.
  2. Davis, M.M. and Bjorkman, P.J. (1988) Nature 334, 395-402.
  3. Vantourout, P. and Hayday, A. (2013) Nat Rev Immunol 13, 88-100.
  4. Kabelitz, D. et al. (2020) Cell Mol Immunol 17, 925-939.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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