WB, IP, IHC-Bond, IHC-P
H
Endogenous
40-45
Rabbit IgG
#B7Z8K6
28526
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
| IHC Leica Bond | 1:100 - 1:400 |
| Immunohistochemistry (Paraffin) | 1:200 - 1:800 |
Storage
For a carrier-free (BSA and azide free) version of this product see product #61129.
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with human TRDC/TCRδ recombinant protein.
Background
The γδ T Cell Receptor (TCR) is a heterodimer composed of a gamma chain and a delta chain expressed on the surface of T cells which participates in antigen recognition (1,2). Binding of gamma-delta TCR to antigen triggers phosphorylation of immunoreceptor tyrosine-based activation motifs (ITAMs) in CD3 chains of the antigen (3). Unlike αβ T cells, human γδ T cells recognize and kill transformed cells independently of human leukocyte antigen (HLA) restriction (3,4). γδ T lymphocytes develop largely in the thymus, generating their defining receptor via RAG-mediated rearrangement of a limited repertoire of variable (V), diversity (D), and joining (J) genes (3). In addition to the T cell receptor, γδ T cells usually also express activating natural killer (NK) receptors, which bind to stress-inducible surface molecules frequently expressed on malignant cells (4).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation IHC-Bond: IHC Leica Bond IHC-P: Immunohistochemistry (Paraffin)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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