WB, IP
H Mk
Endogenous
56
Rabbit IgG
#Q9C035
85363
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro395 of human TRIM5α protein.
Background
TRIM5α is a retroviral restriction factor that was originally identified as an HIV restriction factor in Old World monkeys (1). The restriction specificity of TRIM5α varies between species (2). Human TRIM5α only weakly restricts HIV, but efficiently restricts N-tropic murine leukemia virus (N-MLV) (1-3). TRIM5α is composed of a tripartite motif containing RING, B-box 2, and coiled-coil domains, and a B30.2/SPRY domain (4). A 13 amino acid stretch of the B30.2/SPRY domain containing multiple positively charged residues was found to be essential for viral restriction and responsible for variations across species in restriction specificity (4,5). TRIM5α blocks viral infection by interacting with the incoming viral capsid and promoting its premature disassembly (1,6,7). In addition, TRIM5α, together with UBC13-UEV1A, promotes innate immune signaling by catalyzing the synthesis of K63-linked ubiquitin chains that activate TAK1, AP-1, and NF-κB (8).
- Stremlau, M. et al. (2004) Nature 427, 848-53.
- Hatziioannou, T. et al. (2004) Proc Natl Acad Sci U S A 101, 10774-9.
- Yap, M.W. et al. (2004) Proc Natl Acad Sci U S A 101, 10786-91.
- Javanbakht, H. et al. (2005) J Biol Chem 280, 26933-40.
- Sawyer, S.L. et al. (2005) Proc Natl Acad Sci U S A 102, 2832-7.
- Stremlau, M. et al. (2006) Proc Natl Acad Sci U S A 103, 5514-9.
- Chatterji, U. et al. (2006) J Biol Chem 281, 37025-33.
- Pertel, T. et al. (2011) Nature 472, 361-5.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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