WB
H M R
Endogenous
40-48
Rabbit IgG
#P26651
7538
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala235 of human tristetraprolin protein.
Background
Tristetraprolin (TTP), also known as NUP475, G0S24, RNF162A, TIS11, and ZFP36, is a CCCH tandem zinc-finger protein that binds to adenosine and uridine (AU)-rich elements (AREs) within 3'-untranslated regions of mRNA and leads to their rapid degradation (1-6). Expression of TTP is rapidly induced by mitogens and growth factors including insulin, phorbol ester, cytokines, and lipopolysaccharide (LPS). In addition, numerous phosphorylation sites on TTP can regulate its stability, nuclear to cytosolic trafficking, as well as controlling its ARE-binding activity. Many of the target mRNAs for TTP, such as TNF-α, have critical roles in inflammation and cancer (2), and mice deficient in TTP develop a systemic autoimmune inflammatory syndrome along with excessive TNF-α levels (7). Furthermore, suppression of TTP expression has been identified as a negative prognostic indicator for some cancers (8).
- Brooks, S.A. and Blackshear, P.J. Biochim Biophys Acta 1829, 666-79.
- Sanduja, S. et al. (2012) Front Biosci (Landmark Ed) 17, 174-88.
- Lai, W.S. et al. (1990) J Biol Chem 265, 16556-63.
- DuBois, R.N. et al. (1990) J Biol Chem 265, 19185-91.
- Varnum, B.C. et al. (1989) Oncogene 4, 119-20.
- Heximer, S.P. and Forsdyke, D.R. DNA Cell Biol 12, 73-88.
- Taylor, G.A. et al. (1996) Immunity 4, 445-54.
- Brennan, S.E. et al. (2009) Cancer Res 69, 5168-76.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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