WB
H M R
Endogenous
50
Rabbit IgG
#Q99816
7251
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human TSG101 protein.
Background
Tumor susceptibility gene 101 protein (TSG101) is a multi-domain cytoplasmic protein and fundamental component of ESCRT complex I, which is intimately involved in regulating the trafficking of proteins throughout the endosomal compartment (1). In addition to regulating the sorting of ubiquitinated cargo proteins within the endosomal compartment, TSG101 is involved in regulating diverse biological processes, such as cell proliferation, viral budding and release, and exosome biosynthesis (2-6). While it is clear that TSG101 expression levels are tightly controlled and complete loss of expression causes embryonic lethality due to cell cycle arrest and cell death, the role of TSG101 in malignancy has yet to be fully elucidated (7). Indeed, TSG101 was initially dubbed a tumor suppressor but there are multiple studies that demonstrate an elevated expression in human cancers (8-10).
- Katzmann, D.J. et al. (2001) Cell 106, 145-55.
- Lin, Y.S. et al. (2013) PLoS One 8, e79674.
- Zhong, Q. et al. (1998) Cancer Res 58, 2699-702.
- Oh, H. et al. (2002) Proc Natl Acad Sci U S A 99, 5430-5.
- Théry, C. et al. (2001) J Immunol 166, 7309-18.
- Garrus, J.E. et al. (2001) Cell 107, 55-65.
- Ruland, J. et al. (2001) Proc Natl Acad Sci U S A 98, 1859-64.
- Li, L. et al. (1997) Cell 88, 143-54.
- Oh, K.B. et al. (2007) Oncogene 26, 5950-9.
- Young, T.W. et al. (2007) Clin Cancer Res 13, 3848-54.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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