WB, IP, IHC-P, IF-IC, eCLIP
H M Mk
Endogenous
150, 160
Rabbit IgG
#Q14157
9898
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunohistochemistry (Paraffin) | 1:200 - 1:800 |
| Immunofluorescence (Immunocytochemistry) | 1:50 - 1:200 |
| eCLIP | 1:200 |
For more information about the RBP-eCLIP service please visit Eclipsebio.
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human UBAP2L protein.
Background
Ubiquitin-associated protein 2-like (UBAP2L) is a ubiquitous and highly conserved protein containing an N-terminal ubiquitin-associated (UBA) domain involved in the ubiquitin-proteasome system (UPS) and aggregate formation induced by proteasome inhibitors (1). It can also interact with the Polycomb group protein BMI1 to form a Polycomb subcomplex and regulate hematopoietic stem cell activity (2). UBAP2L is essential for the formation of stress granules (SGs), small cytosolic membraneless organelles that form in eukaryotic cells after stress exposure (3). Some studies suggest that UBAP2L is methylated by the arginine methyltransferase PRMT1, and that this methylation inhibits UBAP2L interaction with SG elements and overall SG assembly (4,5). UBAP2L is also an oncogenic protein found to be amplified and/or overexpressed in a large subset of cancers, including breast cancer, hepatocellular carcinoma, lung adenocarcinoma, glioblastoma, prostate cancer, and colorectal cancer, where it is often associated with tumor growth and poor prognosis (6-12).
- Wilde, I.B. et al. (2011) J Proteome Res 10, 1062-72.
- Bordeleau, M.E. et al. (2014) Blood 124, 2362-9.
- Cirillo, L. et al. (2020) Curr Biol 30, 698-707.e6.
- Maeda, M. et al. (2016) FASEB J 30, 312-23.
- Huang, C. et al. (2020) Cell Death Differ 27, 227-241.
- He, J. et al. (2018) Breast Cancer 25, 224-232.
- Wang, W. et al. (2017) Med Sci Monit 23, 4779-4788.
- Ye, T. et al. (2017) Cell Physiol Biochem 41, 1584-1595.
- Aucagne, R. et al. (2017) FASEB J 31, 5012-5018.
- Zhao, B. et al. (2015) Int J Mol Med 36, 1012-8.
- Li, D. and Huang, Y. (2014) Oncol Rep 32, 1578-84.
- Chai, R. et al. (2016) Tumour Biol 37, 13225-13235.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation IHC-P: Immunohistochemistry (Paraffin) IF-IC: Immunofluorescence (Immunocytochemistry) eCLIP: eCLIP
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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