WB, IP
H Mk
Endogenous
95
Rabbit IgG
#Q96PU4
115426
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala154 of human UHRF2 protein.
Background
Ubiquitin-like PHD and RING finger domain-containing protein 2 (UHRF2), also known as Np95/ICBP90-like RING finger protein (NIRF), is a multi-domain member of the UHRF family and a UHRF1 paralog. Like UHRF1, UHRF2 carries out a variety of biological functions through its multiple domains, including a ubiquitin-like (UBL) domain, tandem Tudor domain (TTD), plant homeodomain (PHD), SET and RING finger-associated (SRA) domain, and really interesting new gene (RING) finger domain (1). Both UHRF1 and UHRF2 localize primarily with highly methylated pericentromeric heterochromatin and specifically recognize H3K9me2/3 marks (2,3). However, although they share high structural and sequence homology, UHRF1 and UHRF2 do not always carry out redundant biological functions. For instance, both UHRF1 and UHRF2 associate with hemi-methylated DNA through their respective SRA domains and complex with DNMT1, but UHRF2 does not target DNMT1 to replication forks and therefore cannot compensate for methylation defects in UHRF1- mouse ES cells (3-5). UHRF1 and UHRF2 also exhibit opposing expression patterns in differentiating cells and various tissues, further suggesting separate functions (6,7). UHRF2 may function as a novel cell cycle regulator by interacting with key proteins such as cyclin A2, CDK2, p53, and PCNA (7,8). Aberrant UHRF2 expression has also been implicated in several cancers, including lung and colorectal cancer as well as hepatocellular carcinoma (9-11).
- Motnenko, A. et al. (2018) PLoS Genet 14, e1007643.
- Papait, R. et al. (2007) Mol Biol Cell 18, 1098-106.
- Zhang, J. et al. (2011) Cell Res 21, 1723-39.
- Sharif, J. et al. (2007) Nature 450, 908-12.
- Zhou, T. et al. (2014) Mol Cell 54, 879-86.
- Pichler, G. et al. (2011) J Cell Biochem 112, 2585-93.
- Mori, T. et al. (2012) FEBS Lett 586, 1570-83.
- Li, Y. et al. (2004) Biochem Biophys Res Commun 319, 464-8.
- Jin, C. et al. (2018) J Cancer 9, 2994-3005.
- Li, L. et al. (2020) Int J Cancer 147, 2239-2252.
- Sun, J. et al. (2021) Int J Med Sci 18, 3097-3105.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.
