WB, IP
H R Mk
Endogenous
25, 28
Rabbit IgG
#Q9BQ65
79650
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:200 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro46 of human USB1 protein.
Background
U6 biogenesis 1 (USB1), also known as MPN1, is a 3’-5’ RNA exonuclease that removes uridines and adenosines from the 3’ end of U6 snRNA, creating a 2’, 3’-cyclic phosphate formation, which protects U6 snRNA from degradation by the nuclear exosome (1-3). USB1 measures the U6 oligo(U) tail by reading the A102 nucleotide position and pauses five uridine residues downstream (3). Loss of USB1 results in a rare condition called poikiloderma with neutropenia, where U6 snRNA is unstable and contains aberrantly long and oligoadenylated 3’ ends, resulting in splicing dysfunction (1,4,5).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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