WB
H
Endogenous
28-65
Rabbit IgG
#Q13336
6563
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala380 of human UT-B/SLC14A1 protein.
Background
Urea transporter B (UT-B, also known as UT1) is encoded by the SLC14A1 gene (1). Urea transporters (UTs) are multipass membrane proteins that transport urea down a concentration gradient. Urea is an organic compound composed of two amino groups and a linked carbamoyl group. Urea is a metabolic waste product of the liver and is excreted via the kidney. UT-B/SLC14A1 was first cloned from human erythrocytes (2). UT-B is not only expressed in endothelial cells of the renal medulla but is also expressed in other organs, including the brain, heart, and pancreas, suggesting broad physiological functions. In the brain, UT-B may be expressed in astrocytes to maintain urea concentrations in the central nervous system. Animals lacking UT-B exhibit depression-like behaviors, suggesting that regulation of urea levels in the brain may be important for proper neuronal function (3).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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