Buy 3 and get the 4th FREE!* | Learn More >>
3120
VASP (A290) Antibody
Primary Antibodies

VASP (A290) Antibody #3120

Reviews ()
Citations (2)

We recommend the following alternatives

# Product Name Application Reactivity
  • WB
H M R Mk
  • WB
  • IP
  • IF
H M R Hm Mk B

Supporting Data

REACTIVITY
SENSITIVITY
MW (kDa) 46, 50
SOURCE Rabbit

Application Key:

  • W-Western
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Storage:

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

VASP (A290) Antibody detects endogenous levels of total VASP protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding the carboxy-terminal sequence of human VASP. Antibodies are purified by peptide affinity chromatography.

Background

Vasodilator-stimulated phosphoprotein (VASP) was originally characterized as a substrate of both cGMP- and cAMP-dependent kinases (PKG and PKA, or cGPK and cAPK, respectively) (1). It is now believed that VASP belongs to the Ena/VASP family of adaptor proteins linking the cytoskeletal system to the signal transduction pathways and that it functions in cytoskeletal organization, fibroblast migration, platelet activation and axon guidance (2,3). Three phosphorylation sites, Ser157, Ser239, and Thr278, have been identified. Ser239 is the major PKG phosphorylation site while Ser157 is the major PKA phosphorylation site (4). Evidence suggests that VASP phosphorylation reduces its association with actin and has a negative effect on actin polymerization (5). Phosphorylation at Ser239 of VASP is a useful marker for monitoring PKG activation and signaling (6,7).

  1. Butt, E. et al. (1994) J Biol Chem 269, 14509-17.
  2. Ball, L.J. et al. (2000) EMBO J. 19, 4903-4914.
  3. Machesky, L.M. (2000) Cell 101, 685-688.
  4. Smolenski, A. et al. (1998) J. Biol. Chem. 273, 20029-20035.
  5. Harbeck, B. et al. (2000) J Biol Chem 275, 30817-25.
  6. Oelze, M. et al. (2000) Circ Res 87, 999-1005.
  7. Lawrence, D.W. and Pryzwansky, K.B. (2001) J Immunol 166, 5550-6.

Pathways & Proteins

Explore pathways + proteins related to this product.

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

To Purchase # 3120