WB, IP
H M R
Endogenous
18
Rabbit IgG
#P62760
7447
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg150 of human Visinin-like Protein 1 protein.
Background
Visinin-like Protein 1, also called VILIP-1 and VSNL1, is a calcium-sensing protein in the central nervous system. Visinin-like Protein 1 exhibits a widespread distribution with high expression in the CNS, and lower expression levels in some peripheral tissues (1). Visinin-like Protein 1 responds to increased intracellular calcium concentration by translocating from the cytoplasm to membranes through calcium-dependent myristoylation at its N-terminus that allows interaction with membranes (2). This change in localization has been proposed to facilitate the activation of compartment-specific signal transduction for the selective activation of downstream signaling cascades (3,4). In Alzheimer’s disease, Visinin-like Protein 1 expression is decreased in the brain, including a reduced number of Visinin-like Protein 1 immunoreactive neurons (5); however, the presence of the protein is increased in the CSF (6), suggesting that it is released from neurons during insults.
- Gierke, P. et al. (2004) Biochem Biophys Res Commun 323, 38-43.
- Kobayashi, M. et al. (1993) J Biol Chem 268, 18898-904.
- Spilker, C. et al. (2002) J Neurosci 22, 7331-9.
- Braunewell, K.H. et al. (1997) J Neurochem 68, 2129-39.
- Bernstein, H.G. et al. (1999) J Neurocytol 28, 655-62.
- Lee, J.M. et al. (2008) Clin Chem 54, 1617-23.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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