Western blot analysis of extracts from various cell lines using VPS26A (E9S7W) Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Expression levels of VPS26A among cell lines are consistent with expectations based on publicly available bioinformatic databases.
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Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody product webpage for recommended antibody dilution.
From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: #12957 Western Blotting Application Solutions Kit
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
* Avoid repeated exposure to skin.
posted June 2005
revised June 2020
Protocol Id: 10
VPS26A (E9S7W) Rabbit mAb recognizes endogenous levels of total VPS26A protein.
Human, Mouse, Rat, Monkey
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro221 of human VPS26A protein.
Vacuolar protein sorting-associated protein 26A (VPS26A), together with VPS29 and VPS35, is part of a trimeric protein complex known as the cargo-selective complex (CSC) (1). The CSC is regarded as the core functional component of the retromer, a multimeric protein complex involved in selective transport of cargo proteins from endosomes to the trans-Golgi network or plasma membrane (2). As part of the CSC, VPS26A does not have intrinsic membrane-binding activity but relies on association with RAB7A for recruitment to the cytosolic face of the endosomal membrane (3,4). Retromer defects are associated with neurological disease, and VPS26A mutations have been linked to perturbed endosomal cargo sorting in atypical parkinsonism (5).
Explore pathways + proteins related to this product.
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