WB, IP, C&R
H Mk
Endogenous
66, 152
Rabbit IgG
#O96028
7468
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
| CUT&RUN | 1:25 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human WHSC1 protein.
Background
Wolf-Hirschhorn syndrome candidate protein 1 (WHSC1), also known as Nuclear SET domain-containing protein 2 (NSD2) and Multiple myeloma SET domain-containing protein (MMSET), is a histone methyltransferase protein that catalyzes the di-methylation of histone H3 Lys36, a mark that is associated with transcriptionally active regions of the genome (1,2). WHSC1 haploinsufficiency is implicated in the developmental disorder known as Wolf-Hirschhorn syndrome (WHS), which is characterized by growth and mental retardation, and congenital heart defects (3). In addition, WHSC1 is overexpressed and associated with poor prognosis in a large variety of human cancers, including neuroblastoma, and ovarian, hepatocellular, endometrial, and colorectal carcinoma (4-8). In multiple myeloma, 15-20% of all patients show overexpression of WHSC1 due to a t(4;14)(p16.3;q32) chromosomal translocation that places the WHSC1 gene upstream of a strong enhancer (2). The WHSC1 gene encodes for three major protein isoforms. Isoform II is the full-length protein containing 1365 amino acids. Isoform I, which is generated by alternative splicing, is identical to the N-terminal 647 amino acids of isoform II. A third isoform, known as REIIBP, is generated from a transcript initiating in intron 9 of the WHSC1 locus and is identical to the C-terminal 584 amino acids of isoform II.
- Vougiouklakis, T. et al. (2015) Epigenomics 7, 863-74.
- Dolloff, N.G. and Talamo, G. (2013) Adv Exp Med Biol 779, 197-221.
- Stec, I. et al. (1998) Hum Mol Genet 7, 1071-82.
- Hudlebusch, H.R. et al. (2011) Clin Cancer Res 17, 2919-33.
- Yang, S. et al. (2013) Biomarkers 18, 257-63.
- Zhou, P. et al. (2013) Pathol Oncol Res 19, 303-9.
- Xiao, M. et al. (2013) J Surg Oncol 107, 428-32.
- Hudlebusch, H.R. et al. (2011) Cancer Res 71, 4226-35.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation C&R: CUT&RUN
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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