| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| CD44 (156-3C11) Mouse mAb | 3570 | 20 µl | 80 kDa | Mouse IgG2a |
| Cyclin D1 (92G2) Rabbit mAb | 2978 | 20 µl | 36 kDa | Rabbit IgG |
| c-Jun (60A8) Rabbit mAb | 9165 | 20 µl | 43, 48 kDa | Rabbit IgG |
| LEF1 (C12A5) Rabbit mAb | 2230 | 20 µl | 25-58 kDa | Rabbit IgG |
| Met (D1C2) XP® Rabbit mAb | 8198 | 20 µl | 140, 170 kDa | Rabbit IgG |
| MMP-7 (D4H5) XP® Rabbit mAb | 3801 | 20 µl | 28, 20-22 kDa | Rabbit |
| c-Myc (D84C12) Rabbit mAb | 5605 | 20 µl | 57-65 kDa | Rabbit IgG |
| TCF1/TCF7 (C63D9) Rabbit mAb | 2203 | 20 µl | 48, 50 kDa | Rabbit IgG |
| Anti-mouse IgG, HRP-linked Antibody | 7076 | 100 µl | Horse | |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat |
Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.
Description
The Wnt/β-Catenin Activated Targets Antibody Sampler Kit provides an economical means to investigate target proteins of the Wnt/β-Catenin signaling pathway. This kit contains enough primary antibody to perform two western blots per primary.
Storage
Background
The Wnt family includes several secreted glycoproteins that play important roles in animal development. β-catenin is a key downstream effector of the Wnt signaling pathway that research studies have shown is implicated in early embryonic development and tumorigenesis in vertebrates (1-3). Following binding of Wnt family proteins to the Frizzled receptor, β-catenin translocates to the nucleus where it interacts with LEF1 and TCF1 to activate canonical targets (4). Accepted canonical targets include CD44, cyclin D1, c-Jun, c-Myc, Met, and MMP-7 (5-11).
- Cadigan, K.M. and Nusse, R. (1997) Genes Dev 11, 3286-305.
- Wodarz, A. and Nusse, R. (1998) Annu Rev Cell Dev Biol 14, 59-88.
- Polakis, P. (1999) Curr Opin Genet Dev 9, 15-21.
- Brantjes, H. et al. (2002) Biol Chem 383, 255-61.
- Zeilstra, J. et al. (2008) Cancer Res 68, 3655-61.
- Shtutman, M. et al. (1999) Proc Natl Acad Sci U S A 96, 5522-7.
- Mann, B. et al. (1999) Proc Natl Acad Sci U S A 96, 1603-8.
- Wilkins, J.A. and Sansom, O.J. (2008) Cancer Res 68, 4963-6.
- Boon, E.M. et al. (2002) Cancer Res 62, 5126-8.
- Brabletz, T. et al. (1999) Am J Pathol 155, 1033-8.
- Clevers, H. and Nusse, R. (2012) Cell 149, 1192-205.
Background References
Trademarks and Patents
Limited Uses
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