The product you are currently viewing is going to be discontinued in the near future. To help you prepare for future experiments, we would like to suggest the following alternative(s).
|12782||XBP-1s (D2C1F) Rabbit mAb||
|27901||XBP-1s (E8C2Z) Mouse mAb||
|40435||XBP-1s (E9V3E) Rabbit mAb||
||H M R|
|47134||XBP-1s (E7M5C) Mouse mAb||
Western blot analysis of extracts from C2C12 cells untreated (-) or treated with tunicamycin (2 μg/ml, 8 hr; +), 3T3 cells untreated (-) or treated with tunicamycin (2 μg/ml, 7 hr; +) and C6 cells untreated (-) or treated with thapsigargin (300 nM, 6 hr; +) using XBP-1s Antibody (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing MycDDK-tagged full-length mouse XBP-1s (Mouse XBP-1s (MycDDK-tagged); +), using XBP-1s Antibody (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), or β-Actin (D6A8) Rabbit mAb #8457 (lower).
|83418S||100 µl||$ 268|
Our US office is closed in observance of Independence Day. We will reopen on Monday, July 6th.
Thank you for your patience.
This product is going to be discontinued in the near future. CST Technical Support is happy to discuss alternatives.
|REACTIVITY||H M R|
|MW (kDa)||60 (human), 55 (mouse)|
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody product webpage for recommended antibody dilution.
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
* Avoid repeated exposure to skin.
posted June 2005
revised June 2020
Protocol Id: 263
XBP-1s Antibody recognizes endogenous levels of total XBP-1s protein.
Human, Mouse, Rat
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala361 of mouse XBP-1s protein. Antibodies are purified by protein A and peptide affinity chromatography.
Following protein synthesis, secretory, intra-organellar, and transmembrane proteins translocate into the endoplasmic reticulum (ER) where they are post-translationally modified and properly folded. The accumulation of unfolded proteins within the ER triggers an adaptive mechanism known as the unfolded protein response (UPR) that counteracts compromised protein folding (1). The transmembrane serine/threonine kinase IRE1, originally identified in Saccharomyces cerevisiae, is a proximal sensor for the UPR that transmits the unfolded protein signal across the ER membrane (2-4). The human homolog IRE1α was later identified and is ubiquitously expressed in human tissues (5). Upon activation of the unfolded protein response, IRE1α splices X-box binding protein 1 (XBP-1) mRNA through an unconventional mechanism using its endoribonuclease activity (6). This reaction converts XBP-1 from an unspliced XBP-1u isoform to the spliced XBP-1s isoform, which is a potent transcriptional activator that induces expression of many UPR responsive genes (6).
Explore pathways + proteins related to this product.
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