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Xenophagy Antibody Sampler Kit

1 Kit

(9 x 20 microliters)

CST Logo
Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

Web:

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Product Includes Product # Quantity Mol. Wt Isotype/Source
LRSAM1 (D1O5S) Rabbit Monoclonal Antibody2840520 µl84 kDaRabbit IgG
Galectin-3/LGALS3 (E7B6R) Rabbit Monoclonal Antibody8957220 µl28 kDaRabbit IgG
NDP52 (D1E4A) Rabbit Monoclonal Antibody6073220 µl52, 60 kDaRabbit IgG
TAX1BP1 (D1D5) Rabbit Monoclonal Antibody510520 µl92 kDaRabbit IgG
Atg16L1 (D6D5) Rabbit Monoclonal Antibody808920 µl66, 68 kDaRabbit IgG
Phospho-Atg16L1 (Ser278) (E7K6H) Rabbit Monoclonal Antibody4551120 µl68 kDaRabbit IgG
Phospho-TBK1/NAK (Ser172) (D52C2) Rabbit Monoclonal Antibody548320 µl84 kDaRabbit IgG
TBK1/NAK (E8I3G) Rabbit Monoclonal Antibody3806620 µl84 kDaRabbit IgG
LC3B (E7X4S) Rabbit Monoclonal Antibody4356620 µl14, 16 kDaRabbit IgG
Anti-rabbit IgG, HRP-linked Antibody7074100 µlGoat

Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.

Description

The Xenophagy Antibody Sampler Kit provides an economical means of detecting selected targets involved in the process of xenophagy. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibodies.

Background

Xenophagy provides an important defense against foreign pathogens, such as bacteria and viruses, by targeting them for degradation through autophagy (1-3). Several well-known pathogens can be targeted for degradation through xenophagy, including Salmonella typhimurium, Streptococcus pyogenes, and Mycobacterium tuberculosis, and loss of core autophagy genes can result in enhanced vulnerability to these infections. Pathogens are generally targeted to LC3B or other Atg8 family members localized on autophagosome membranes. Sequestosome 1/p62-like receptors (SLRs) function as cargo receptors through ubiquitin-mediated interactions along with LIR-domain interactions with Atg8 proteins on the autophagosome. The SLR TAX1BP1 is recruited to ubiquitylated Salmonella and plays a key role in xenophagy (4). Another SLR NDP52 has also been reported to be required for xenophagy of several pathogens (5,6). LRSAM1 is an E3 ubiquitin ligase with a leucine-rich repeat recognized by microbial ligands and recruits NDP52 (7). In addition, galectins are a family of cytosolic lectins that are associated with phagophores and bacteria. Galectin-3 is recruited to bacterial membranes and is important for protection against Mycobacterium tuberculosis (8). TBK1 is a serine/threonine kinase that plays a major role in controlling autophagy by targeting substrates in the SLR family and the maturation of autophagosomes. TBK1 is phosphorylated at Ser172 within its activation loop, which is required for its kinase activity (9). Atg16L1 is a component of the Atg12-Atg5 conjugation system required for autophagosome maturation (10,11). ULK1-mediated phosphorylation of Atg16L1 at Ser278 promotes xenophagy (12).

Background References

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] • Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.