WB, IP
H
Endogenous
40
Rabbit IgG
#O43542
7517
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val280 of human XRCC3 protein.
Background
XRCC3 (X-ray repair cross-complementing protein 3) is one of five RAD51 paralogs that form two distinct complexes, BCDX2 (RAD51B, RAD51C, RAD51D, and XRCC2) and CX3 (RAD51C and XRCC3) (1). Along with RAD51, these complexes are crucial in the repair of DNA double-strand breaks through homologous recombination (2), and in the response to DNA replication stress (3,4). The CX3 complex also plays a role in maintaining mitochondrial DNA integrity during replicative stress (5). XRCC3 genetic polymorphisms are associated with risk and prognosis in human cancer (6-9).
- Masson, J.Y. et al. (2001) Genes Dev 15, 3296-307.
- Thacker, J. (2005) Cancer Lett 219, 125-35.
- Rein, H.L. et al. (2021) Curr Opin Genet Dev 71, 86-91.
- Berti, M. et al. (2020) Nat Commun 11, 3531.
- Mishra, A. et al. (2018) Mol Cell Biol 38, e00489-17. doi: 10.1128/MCB.00489-17.
- Zhao, Y. et al. (2019) J Oncol 2019, 2408946.
- Cheng, S. et al. (2015) Int J Clin Exp Pathol 8, 1911-9.
- Bei, L. et al. (2015) Bull Cancer 102, 332-9.
- Ozoran, E. et al. (2022) Int J Breast Cancer 2022, 5817841.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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