WB, IP
H
Endogenous
44, 58
Rabbit
#Q9H171
81030
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln372 of human ZBP1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
ZBP1 (Z-DNA binding protein 1), also referred to as DAI (DNA-dependent activator of IFN-regulatory factors) and DLM-1, is a nucleotide binding protein that plays a role in tumorigenesis and innate immune responses to viral infection (1). It is expressed at high levels in lymphatic tissues and intestine and is induced in macrophages by interferon gamma or by LPS (2,3). It contains two amino-terminal Z-alpha domains that bind to left-handed Z-DNA and Z-RNA (4,5). In addition, an adjacent domain binds right-handed B-DNA that allows for it to function as a cytosolic DNA sensor in innate immune responses, triggering activation of TBK1 and IRF-3, and subsequent production of type I interferons (6,7). Furthermore, ZBP1 can trigger the activation of NF-κB through interaction with the RIP homotypic interaction motif (RHIM) of RIPK1 and RIPK3 (8). ZBP1 binding to RIPK3 can also induce a process of programmed necrosis termed necroptosis (9). In contrast, its interaction with RIPK1 can repress necroptosis (10,11). The mRNA binding activity of ZBP1 is also thought to play a role in tumorigenesis. ZBP1 is repressed in metastatic breast cancer, which leads to dysregulation of mRNA targets involved in proliferation and metastasis (12,13).
- Kuriakose, T. and Kanneganti, T.D. (2018) Trends Immunol 39, 123-34.
- Rothenburg, S. et al. (2002) Nucleic Acids Res 30, 993-1000.
- Fu, Y. et al. (1999) Gene 240, 157-63.
- Ross, A.F. et al. (1997) Mol Cell Biol 17, 2158-65.
- Schwartz, T. et al. (2001) Nat Struct Biol 8, 761-5.
- Takaoka, A. et al. (2007) Nature 448, 501-5.
- Wang, Z. et al. (2008) Proc Natl Acad Sci USA 105, 5477-82.
- Rebsamen, M. et al. (2009) EMBO Rep 10, 916-22.
- Upton, J.W. et al. (2012) Cell Host Microbe 11, 290-7.
- Newton, K. et al. (2016) Nature 540, 129-33.
- Lin, J. et al. (2016) Nature 540, 124-8.
- Gu, W. et al. (2009) J Cell Sci 122, 1895-905.
- Wang, G. et al. (2016) Oncotarget 7, 15690-702.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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