WB, IP, IF-IC
H
Endogenous
65, 130
Rabbit IgG
#Q9C0K1
64116
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:50 |
Immunofluorescence (Immunocytochemistry) | 1:1600 - 1:3200 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala265 of human ZIP8/SLC39A8 protein.
Background
Zinc transporter zinc-and iron-related protein 8 (ZIP8) is encoded by the SLC39A8 gene and is a member of the solute carrier gene (SLC) superfamily (1). As a membrane protein, ZIP8 functions to transport manganese, cadmium, and zinc across the plasma membrane (2). Metal ions are essential elements that are required cofactors for proteins, including enzymes and transcription factors. Maintenance of proper metal ion homeostasis is required for normal mammalian tissue development and function. Mice lacking SLC39A8 reveal a critical role of ZIP8 in organogenesis in multiple tissues, including the brain and kidney, during development (3,4). Mutations in SLC39A8 are linked to autosomal-recessive forms of intellectual disability, suggesting that altered ZIP8-dependent metal ion regulation may contribute to human neuronal developmental diseases (5).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation IF-IC: Immunofluorescence (Immunocytochemistry)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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