WB, IP, IF-IC, ChIP
H
Endogenous
140
Rabbit IgG
#O75362
7764
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunofluorescence (Immunocytochemistry) | 1:800 - 1:1600 |
| Chromatin IP | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ZNF217 protein.
Background
Zinc finger protein 217 (ZNF217) is an oncogenic transcription factor that regulates the expression of a wide variety of genes. ZNF217 is a member of the Kruppel-like factor (KLF) family of transcription factors, which consists of zinc finger proteins that bind to CACCC or GT box DNA elements (1). ZNF217 regulates the expression of its target genes through direct DNA binding, and through interaction with transcriptional co-repressors in the CoREST-HDAC complex (2). Although it was initially described as a transcriptional repressor, ZNF217 may also act as a transcriptional activator depending on the target gene (3). Amplification of the ZNF217 gene itself has been observed in a variety of tumors, where it is typically associated with poor prognosis (4-7). Overexpression of ZNF217 protein leads to increased cell proliferation and promotes tumor growth in breast and ovarian cancer (8,9). ZNF217 also interferes with apoptotic signaling and confers resistance to chemotherapeutic drugs in breast cancer (9,10).
- Pollak, N.M. et al. (2018) JACC Basic Transl Sci 3, 132-156.
- Cowger, J.J. et al. (2007) Oncogene 26, 3378-86.
- Cohen, P.A. et al. (2015) Oncotarget 6, 41566-81.
- Rooney, P.H. et al. (2004) J Pathol 204, 282-8.
- Rahman, M.T. et al. (2012) Cancer 118, 2846-57.
- Rahman, M.T. et al. (2012) Anticancer Res 32, 3091-5.
- Mao, X.G. et al. (2011) Lab Invest 91, 1068-78.
- Li, J. et al. (2014) Int J Clin Exp Pathol 7, 3038-47.
- Thollet, A. et al. (2010) Mol Cancer 9, 291.
- Huang, G. et al. (2005) Hum Mol Genet 14, 3219-25.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation IF-IC: Immunofluorescence (Immunocytochemistry) ChIP: Chromatin IP
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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