1M Ammonium bicarbonate buffer in HPLC water is provided for use with Cell Signaling Technology's patented PTMScan® protocol. Secondary trypsin digestion of enriched LysC or Methylated peptides is recommended for all absophillic and methylation-specific motif antibodies. Use this 1M ammonium bicarbonate (20X) stock to prepare the final digestion buffer of 50mM ammonium bicarbonate with 5% acetonitrile. Consult the specific PTMScan® kit and protocol for more details on the appropriate protease before digesting any protein samples.
Aliquoted buffer can be stored at -80°C for long-term storage for 12 months.
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