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38572
PTMScan®HS Phospho-Tyrosine (P-Tyr-1000) Kit
Proteomic Analysis Products
PTMScan

PTMScan®HS Phospho-Tyrosine (P-Tyr-1000) Kit #38572

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PTMScan®HS Phospho-Tyrosine (P-Tyr-1000) Kit: Image 1
Motif analysis using all phospho-tyrosine peptides enriched and identified by PTMScan® HS Phospho-Tyrosine (P-Tyr-1000) Kit from two different samples. Peptides from mouse embryo and MKN-45 human gastric cancer cells were immunoprecipitated with PTMScan® HS pY Magnetic Immunoaffinity Beads in seven independent experiments. Orbitrap Fusion Lumos mass spectrometer analysis identified a total of 3,620 unique sites. The motif logo shows that the pY antibody is a general motif antibody that recognizes the pY motif independent of protein context, without other amino acid preferences.
Product Includes Volume (with Count)
PTMScan® HS Phospho-Tyrosine (P-Tyr-1000) Magnetic Immunoaffinity Beads 1 x 200 µl
PTMScan® HS Immunoaffinity Purification (IAP) Bind Buffer #1 (1X) 1 x 25 ml
PTMScan® HS Immunoaffinity Purification (IAP) Wash Buffer (1X) 1 x 50 ml
PTMScan® Limited Use License 1 x 1 ea

Storage

All components in this kit are stable for at least 12 months when stored at the recommended temperature. Do not aliquot the antibody.

Product Description

PTMScan® HS is an enhanced PTMScan® methodology with improved identification of post-translationally modified peptides. PTMScan® technology employs a proprietary methodology from Cell Signaling Technology (CST) for peptide enrichment by immunoprecipitation using a specific bead-conjugated antibody in conjunction with liquid chromatography tandem mass spectrometry (LC-MS/MS) for quantitative profiling of post-translational modification (PTM) sites in cellular proteins. PTMs that can be analyzed by PTMScan® technology include phosphorylation, ubiquitination, acetylation, and methylation, among others. The technology enables researchers to isolate, identify, and quantitate large numbers of post-translationally modified cellular peptides with a high degree of specificity and sensitivity (HS), providing a global overview of PTMs in cell and tissue samples without bias about where the modified sites occur. For more information on PTMScan® products and services, please visit www.cellsignal.com/applications/proteomics.

Background

Tyrosine phosphorylation plays a key role in cellular signaling (1). Research studies have shown that in cancer, unregulated tyrosine kinase activity can drive malignancy and tumor formation by generating inappropriate proliferation and survival signals (2). Antibodies specific for phospho-tyrosine (3,4) have been invaluable reagents in these studies. The phospho-tyrosine monoclonal antibodies developed by Cell Signaling Technology are exceptionally sensitive tools for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery.
  1. Schlessinger, J. (2000) Cell 103, 211-25.
  2. Blume-Jensen, P. and Hunter, T. (2001) Nature 411, 355-65.
  3. Ward, S.G. et al. (1992) J Biol Chem 267, 23862-9.
  4. Glenney, J.R. et al. (1988) J Immunol Methods 109, 277-85.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
ProtiFi and S-Trap are trademarks of ProtiFi, LLC.
PTMScan is a trademark of Cell Signaling Technology, Inc.
OrbiTrap is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at [email protected] For information regarding commercial licensing terms please contact CST Pharma Services Department at [email protected]