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86506
PTMScan® Lysine/Arginine Modification Antibody Sampler Kit
Proteomic Analysis Products

PTMScan® Lysine/Arginine Modification Antibody Sampler Kit #86506

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The Motif Logo was generated from a MethylScan® LC-MS/MS experiment using 722 nonredundant tryptic peptides derived from human HCT 116 cells immunoprecipitated with immunoaffinity beads from PTMScan® Mono-Methyl Arginine motif [mme-RG] Kit #12235. The logo represents the relative frequency of amino acids in each position surrounding the central methylated arginine residue. Glycine residues are enriched, especially at the +1 position, in the context of mono-methylated arginine when compared to the overall expected frequency in the human proteome. Of the total methylated arginine peptides, 68% contain the [mme-RG] motif.

The Motif Logo was generated from an AcetylScan® LC-MS/MS experiment using 2549 nonredundant tryptic peptides derived from mouse liver tissue immunoprecipitated with immunoaffinity beads from PTMScan® Acetyl-Lysine Motif [Ac-K] Kit #13416. The logo represents the relative frequency of amino acids in each position surrounding the central acetylated lysine residue within this data set.

This Motif Logo was generated from a MethylScan® LC-MS/MS experiment using 169 nonredundant tryptic peptides derived from mouse embryo and immunoprecipitated with immunoaffinity beads from PTMScan® Asymmetric Di-Methyl Arginine Motif [adme-R] Kit #13474. The logo represents the relative frequency of amino acids in each position surrounding the central asymmetric di-methyl arginine.

The Motif Logo was generated from a MethylScan® LC-MS/MS experiment using 95 nonredundant tryptic peptides derived from mouse embryo immunoprecipitated with immunoaffinity beads from PTMScan® Symmetric Di-Methyl Arginine Motif [sdme-RG] Kit #13563. The logo represents the relative frequency of amino acids in each position surrounding the central symmetrically di-methylated arginine residue. Glycine residues are enriched, especially at the +1 and -1 positions, in the context of symmetric di-methyl arginine when compared to the overall expected frequency in the mouse proteome.

The Motif Logo was generated from a MethylScan® LC-MS/MS experiment using 130 nonredundant tryptic peptides derived from mouse liver tissue immunoprecipitated with immunoaffinity beads from PTMScan® Pan-Methyl Lysine Kit #14809. The logo represents the relative frequency of amino acids in each position surrounding the central methylated lysine residue within this data set.

Product Usage Information

Cells are lysed in a urea-containing buffer, cellular proteins are digested by proteases, and the resulting peptides are purified by reversed-phase solid-phase extraction. Peptides are then subjected to immunoaffinity purification using a PTMScan® Motif Antibody conjugated to protein A agarose beads. Unbound peptides are removed through washing, and the captured PTM-containing peptides are eluted with dilute acid. Reversed-phase purification is performed on microtips to desalt and separate peptides from antibody prior to concentrating the enriched peptides for LC-MS/MS analysis. CST recommends the use of PTMScan® IAP Buffer #9993 included in the kit. A detailed protocol and Limited Use License allowing the use of the patented PTMScan® method are included with the kit.

Storage:

Antibody beads supplied in IAP buffer containing 50% glycerol. Store at -20°C. Do not aliquot the antibodies.

Product Description

PTMScan® Technology employs a proprietary methodology from Cell Signaling Technology (CST) for peptide enrichment by immunoprecipitation using a specific bead-conjugated antibody in conjunction with liquid chromatography (LC) tandem mass spectrometry (MS/MS) for quantitative profiling of post-translational modification (PTM) sites in cellular proteins. These include phosphorylation (PhosphoScan®), ubiquitination (UbiScan®), acetylation (AcetylScan®), and methylation (MethylScan®), among others. PTMScan® Technology enables researchers to isolate, identify, and quantitate large numbers of post-translationally modified cellular peptides with a high degree of specificity and sensitivity, providing a global overview of PTMs in cell and tissue samples without preconceived biases about where these modified sites occur (1). For more information on PTMScan® Proteomics Services, please visit www.cellsignal.com/services/index.html.

Background

For more information on our PTMScan® Motif Antibody Kits please visit www.cellsignal.com. Background information specific to each component in the sampler kit can be found on the individual webpages. Acetylation of lysine is described on the webpage for PTMScan® Acetyl-Lysine Motif [Ac-K] Kit #13416. Methylation of lysine is described on the webpage for PTMScan® Pan-Methyl Lysine Kit #14809. Methylation of arginine in its various types is described on the webpages for PTMScan® Mono-Methyl Arginine Motif [mme-RG] Kit #12235, PTMScan® Symmetric Di-Methyl Arginine Motif [sdme-RG] Kit #13563, and PTMScan® Asymmetric Di-Methyl Arginine Motif [adme-R] Kit #13474.

  1. Rush, J. et al. (2005) Nat Biotechnol 23, 94-101.
For Research Use Only. Not For Use In Diagnostic Procedures.

AcetylScan is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
MethylScan is a trademark of Cell Signaling Technology, Inc.
PTMScan is a trademark of Cell Signaling Technology, Inc.
UbiScan is a trademark of Cell Signaling Technology, Inc.
U.S. Pat. No. 9,085,609
Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at ptmscan@cellsignal.com.