|Product Includes||Volume (with Count)|
|PTMScan® Ubiquitin Branch Motif (K-ε-GG) Immunoaffinity Beads||3 x 80 µl|
|PTMScan® IAP Buffer (10X) 9993||3 x 600 µl|
|PTMScan® Trypsin Digested Control Peptides I 12219||1 x 1 vial|
|PTMScan® Limited Use License||1 x 1 ea|
Cells are lysed in a urea-containing buffer, cellular proteins are digested by proteases, and the resulting peptides are purified by reversed-phase solid-phase extraction. Peptides are then subjected to immunoaffinity purification using a PTMScan® Motif Antibody conjugated to protein A agarose beads. Unbound peptides are removed through washing, and the captured PTM-containing peptides are eluted with dilute acid. Reversed-phase purification is performed on microtips to desalt and separate peptides from antibody prior to concentrating the enriched peptides for LC-MS/MS analysis. CST recommends the use of PTMScan® IAP Buffer #9993 included in the kit. A detailed protocol and Limited Use License allowing the use of the patented PTMScan® method are included with the kit.
PTMScan® Trypsin Digested Control Peptides I are supplied as lyophilized powder. Centrifuge the tube containing the lyophilized peptide in order to collect all material to be dissolved. Add 1.4 ml PTMScan® IAP Buffer (1X) and follow the PTMScan® kit protocol from the Immunoaffinity Purification (IAP) step using the entire vial of reconstituted peptides.
Antibody beads are supplied in IAP buffer containing 50% glycerol. Store at -20°C. Do not aliquot the antibody. Store lyophilized peptides at -20°C. In lyophilized form, the peptides are stable for 24 months.
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