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72969
PTMScan® Trypsin, Mass Spectrometry Grade
Proteomic Analysis Products
Miscellaneous

PTMScan® Trypsin, Mass Spectrometry Grade #72969

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PTMScan® Trypsin, Mass Spectrometry Grade: Image 1

Product Usage Information

PTMScan® Trypsin, Mass Spectrometry Grade is provided for use with Cell Signaling Technology's patented PTMScan® protocol in the Trypsin Digestion of Enriched Peptides step. Reconstitute the lyophilized trypsin with 40ul of mass spectrometry grade water to make a 0.5 ug/ul stock. Use immediately or aliquot and store at -80°C. Consult the specific PTMScan® kit and protocol for more details.

Storage

Store lyophilized trypsin powder at -20ºC protected from moisture. Once reconstituted, store trypsin solutions at -80ºC. Lyophilized trypsin has a shelf life of 1 year at -20ºC and solutions are stable for 6 months at -80ºC.

Product Description

Trypsin is a serine endopeptidase derived from its inactive pancreatic zymogen, trypsinogen, when the N-terminal 6 amino acid leader sequence is enzymatically removed. Activated trypsin cleaves amide and ester bonds of lysine and arginine and this mass spectrophotometry grade Trypsin is intended for use in protein sequencing, and proteomics applications.

Background

Trypsin digests polypeptides by hydrolysis at the carboxyl side of unmodified arginine and lysine residues (1). Proteolysis is slower when the cleavage site is flanked by acidic residues and will not occur if the lysine or arginine is followed by a proline (2-5). Trypsin's activity is optimal at pH 8.0 and is inhibited to varying degrees by organophosphorus compounds such as diisopropyl fluorophosphate, as well as EDTA, aprotinin, Ag+, and benzamidine (6-8).

  1. Brown, W.E. and Wold, F. (1973) Biochemistry 12, 828-34.
  2. Rodriguez, J. et al. (2008) J Proteome Res 7, 300-5.
  3. Leiros, H.K. et al. (2004) Protein Sci 13, 1056-70.
  4. Rawlings, N.D. and Barrett, A.J. (1994) Methods Enzymol 244, 19-61.
  5. Perona, J.J. and Craik, C.S. (1995) Protein Sci 4, 337-60.
  6. Kostka, V. and Carpenter, F.H. (1964) J Biol Chem 239, 1799-803.
  7. Levilliers, N. et al. (1970) Arch Biochem Biophys 140, 474-83.
  8. Polgár, L. (2005) Cell Mol Life Sci 62, 2161-72.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PTMScan is a trademark of Cell Signaling Technology, Inc.
To Purchase # 72969S
Product # Size Price
72969P
20 µg  (20 ug) $ 26
72969S
100 µg  (5 x 20 µg) $ 113