Western blot titration of NIH-3T3 cells using p44/42 MAPK (Erk1/2) (3A7) Mouse mAb #9107 and #7056 as the secondary antibody.
Affinity purified goat anti-mouse IgG (H&L) antibody is conjugated to calf intestinal alkaline phosphatase. This product has been optimized for use as a secondary antibody in Western blotting and ELISA applications.
Recommended Antibody Dilutions:
Western blotting 1:1000-1:3000
Store at -20°C.
The alkaline phosphatase (AP) conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent or other substrates for detection on western blots. One of the advantages of AP conjugation is that the reaction rate remains linear for a long period of time.
This product detects both heavy and light chain (AP-anti-mouse-IgG (H+L), affinity purified.
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