Anti-mouse IgG (H+L), F(ab')2 Fragment (Sepharose® Bead Conjugate) (#5946) Datasheet Without Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:

REACTIVITY:

SENSITIVITY:

MW (kDa):

Source/Isotype:

Rabbit

Product Information

Product Usage Information

Add 10-20 μl of well-vortexed beads to 200 μl of cell lysate at 1 mg/ml in 1X Cell Lysis Buffer (10X) #9803. See protocol for more details.

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol. Store at –20°C. Do not aliquot the antibodies.

Specificity / Sensitivity

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, mouse IgG and mouse serum. No reaction was observed against anti-pepsin, anti-goat IgG F_c, or human serum proteins.

Species Reactivity:

Mouse

Source / Purification

The Anti-mouse IgG (H+L), F(ab')₂ Fragment is produced by immunizing goats with mouse IgG whole molecules. The Anti-mouse IgG (H+L), F(ab')₂ Fragment is prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption to remove any unwanted reactivities, pepsin digestion, and chromatographic separation.

Product Description

This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated sepharose beads. Anti-mouse IgG (H+L), F(ab')₂ Fragment (Sepharose Bead Conjugate) is useful for the immunoprecipitation of antibodies raised in mice.

Background

Anti-mouse IgG (H+L), F(ab')₂ Fragment (Sepharose Bead Conjugate) is ideal for immunoprecipitation. This secondary antibody will detect mouse antibodies. Since this secondary antibody is made with F(ab')₂ fragments, non-specific binding through F_c receptors present on cells will be eliminated.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Applications Key

IP: Immunoprecipitation

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

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