Flow cytometric analysis of Jurkat cells using Akt (pan) (C67E7) Rabbit mAb #4691 detected with Anti-rabbit IgG (H+L), F(ab')2 Fragment (PE Conjugate) (solid line) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control
#3900 (dashed line).
Anti-rabbit IgG (H+L), F(ab')2 Fragment was conjugated to phycoerythrin (PE) under optimal conditions. This F(ab')2 fragment product results in less non-specific binding, as it lacks the Fc domain that can bind to the cells with Fc receptors.
The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution of 1:250 - 1:1000 should yield acceptable results for flow cytometry assays.
Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
F(ab')2 fragments are prepared from goat antibodies that have been affinity purified and shown no reactivity against non-immunoglobulin rabbit serum proteins, serum proteins from bovine, human, or mouse and IgG from human or mouse based on immunoelectrophoresis (IEP) testing.
This product has been optimized for use as a secondary antibody in flow cytometry. Fluorescent anti-species IgG conjugates are ideal for flow cytometry and immunofluorescence. Cell Signaling Technology’s strict quality control procedures assure that each conjugate provides optimal specificity and fluorescence.