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14705
Anti-Rabbit IgG (H+L), (PE Conjugate)
Secondary Antibodies

Anti-Rabbit IgG (H+L), (PE Conjugate) #14705

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# Product Name Application Reactivity
  • F

Supporting Data

REACTIVITY
SENSITIVITY
MW (kDa)
Isotype Goat 

Application Key:

  • W-Western
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Description

Affinity purified goat anti-rabbit IgG (H+L) antibody is conjugated to R-phycoerythrin. This product has been optimized for use as a secondary antibody in flow applications. Excitation is 450-580 nm and peak emission is 590 nm.

Product Usage Information

The optimal dilution of the secondary antibody should be determined for each application. However, a final dilution of 1:500 should yield acceptable results for most flow cytometry applications.

Storage:

Supplied in 10 mM HEPES, 150 mM NaCl, pH 7.4, 0.08% sodium azide. Store at 4°C. Protect from light for up to one year. Do not freeze.

Specificity / Sensitivity

Anti-Rabbit IgG (H+L), (PE Conjugate) reacts with heavy and light chain of most rabbit immunoglobulins. No cross-reactivity to other serum proteins has been detected.

Less than 1% cross-reactivity is observed with immunoglobulins from other species.

Background

This product has been optimized for use as a secondary antibody in flow cytometry applications. Cell Signaling Technology’s strict quality control procedures assure that each conjugate provides optimal specificity and fluorescence.

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.

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