Recommended Antibody Dilutions:
1:1000 - 1:3000
Recommended antibody dilutions are provided in ranges because the optimal dilution is dependent on many factors, such as antigen density and permeability.
Supplied in 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease- Free) and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody product webpage for recommended antibody dilution.
From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: #12957 Western Blotting Application Solutions Kit
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
* Avoid repeated exposure to skin.
posted June 2005
revised June 2020
Protocol Id: 19
Goat Anti-Mouse IgG, Light-Chain Specific Antibody (HRP Conjugate) is produced by immunizing goats with mouse IgG. The Anti-Mouse IgG, Light-Chain Specific Antibody is purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
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