Revision 5
Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected] cellsignal.com

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:

C&T

REACTIVITY:

Rab

SENSITIVITY:

MW (kDa):

Source/Isotype:

Goat 

Product Information

Product Usage Information

The CUT&Tag dilution was determined using CUT&Tag Assay Kit #77552. Recommended antibody dilution is 1:50.

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 2 mg/mL bovine serum albumin (BSA), and 50% glycerol. Store at –20°C. Do not aliquot the antibody. This product is stable for 24 months when stored at -20°C.

Specificity / Sensitivity

Goat Anti-Rabbit IgG (H+L) Antibody recognizes native rabbit IgG.

Species Reactivity:

Rabbit

Source / Purification

The purified antibody was isolated from goat sera and prepared by affinity chromatography.

Product Description

Designed for use with rabbit polyclonal and monoclonal antibodies, this affinity purified goat anti-rabbit IgG is thoroughly validated with Cell Signaling Technology® primary antibodies and will work optimally with the Cell Signaling Technology® CUT&Tag protocol, ensuring accurate and reproducible results.

Background

Goat Anti-Rabbit IgG (H+L) Antibody is a secondary antibody designed to bind to primary antibodies generated from the rabbit species. It is used to detect and amplify signals in various assays.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Applications Key

C&T: CUT&Tag

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Revision 5
#35401

Goat Anti-Rabbit IgG (H+L) Antibody

CUT and Tag Image 1: Goat Anti-Rabbit IgG (H+L) Antibody Expand Image
CUT&Tag was performed with HCT 116 cells and Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb #9751 and Goat Anti-Rabbit IgG (H+L) Antibody, using CUT&Tag Assay Kit #77552. DNA library was prepared using CUT&Tag Dual Index Primers and PCR Master Mix for Illumina Systems #47415. The figure shows binding across GAPDH, a known target gene of H3K4me3 (see our #9751 ChIP-qPCR figure).
CUT and Tag Image 2: Goat Anti-Rabbit IgG (H+L) Antibody Expand Image
CUT&Tag was performed with HCT 116 cells and Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb #9751 and Goat Anti-Rabbit IgG (H+L) Antibody, using CUT&Tag Assay Kit #77552. DNA library was prepared using CUT&Tag Dual Index Primers and PCR Master Mix for Illumina Systems #47415. The figures show binding across chromosome 12 (upper), including GAPDH (lower), a known target gene of H3K4me3 (see our #9751 ChIP-qPCR figure).