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3677
Mouse Anti-rabbit IgG (Light-Chain Specific) (L57A3) mAb
Secondary Antibodies

Mouse Anti-rabbit IgG (Light-Chain Specific) (L57A3) mAb #3677

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Citations (3)

Supporting Data

REACTIVITY
SENSITIVITY
MW (kDa) 25
Isotype Mouse 

Application Key:

  • W-Western
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Description

Affinity purified mouse anti-rabbit IgG (Light-Chain Specific) antibody. This product has been optimized for use as a secondary antibody in Western blotting applications.

Storage:

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Mouse Anti-rabbit IgG (Light-Chain Specific) (L57A3) mAb recognizes the light chain of rabbit IgG, either in native conformation or denatured and reduced form. It does not bind to the denatured and reduced rabbit IgG heavy chain (about 50 kDa) on western blot.

Source / Purification

Monoclonal antibody is produced by immunizing animals with native total rabbit IgG.

Background

Mouse Anti-rabbit IgG (Light-Chain Specific) (L57A3) mAb only reacts with the light chain of rabbit IgG and does not bind to the denatured and reduced rabbit IgG heavy chain. When performing immunopreciptiation (IP) followed by western blotting, the denatured rabbit IgG heavy chain of the primary antibody used for IP runs at approximately 50 kDa on the subsequent western blot and can often obscure bands of proteins that have a similar molecular weight. Using Mouse Anti-Rabbit IgG (Light-Chain Specific) (L57A3) mAb as a secondary antibody will eliminate this problem.

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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