| Product Includes | Item # | Volume | Reactivity | Isotype |
|---|---|---|---|---|
| CD11c (D3V1E) XP® Rabbit mAb (SignalStar™ Conjugate 0017) | 94363 | 50 µl | H | Rabbit IgG |
| Complementary Oligo (CO-0017-594) | 26764 | 22 µl |
Storage
Description
SignalStar multiplex immunohistochemistry (IHC) is an advanced technology for labeling multiple proteins simultaneously in tissue samples using specific primary antibodies and fluorescent detection reagents. This technology offers accuracy and reliability in visualizing and analyzing protein expression while maintaining spatial context and tissue architecture.
SignalStar Oligo-Antibody Pairs are compatible with the SignalStar Multiplex IHC Buffer Kits for use in fluorescent multiplex imaging experiments. This product includes the oligo-conjugated antibodies and complementary oligos required for labeling your target protein on up to 10 slides. SignalStar Multiplex IHC Buffer Kits are required to amplify and image the target signal. Multiple oligo-antibody pairs can be conveniently combined into a multiplex panel using the SignalStar Multiplex IHC Panel Builder. SignalStar Multiplex IHC Kits & Reagents are not compatible with all of Cell Signaling Technology® products and protocols that are recommended for use in immunohistochemical assays.
Specificity/Sensitivity
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant human CD11c protein.
Background
CD11c (integrin αX, ITGAX) is a transmembrane glycoprotein that forms an α/β heterodimer with CD18 (integrin β2), which interacts with a variety of extracellular matrix molecules and cell surface proteins (1). CD11c is primarily used as a dendritic cell marker. Dendritic cells can be classified into two major types: CD11c+ conventional dendritic cells that specialize in antigen presentation, and CD11c- plasmacytoid dendritic cells that specialize in type I interferon production (2, 3). CD11c expression has also been observed on activated NK cells, subsets of B cells, monocytes, granulocytes, and some B cell malignancies including hairy cell leukemia (4-7).
- Uotila, L.M. et al. (2013) J Biol Chem 288, 33494-9.
- Kohrgruber, N. et al. (1999) J Immunol 163, 3250-9.
- Siegal, F.P. et al. (1999) Science 284, 1835-7.
- Racine, R. et al. (2008) J Immunol 181, 1375-85.
- Werfel, T. et al. (1991) J Immunol 147, 2423-7.
- Cabañas, C. et al. (1988) Hybridoma 7, 167-76.
- Kristensen, J.S. et al. (1987) Blood 70, 1063-8.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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