Revision 1

#31774

Store at -20C

E-Cadherin (24E10) & CO-0103-647 SignalStar Oligo-Antibody Pair

1 Kit

(10 slides)

UniProt ID:
#P12830

Entrez-Gene Id:
#999

CST Logo
Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Product Includes Product # Volume Reactivity Isotype
E-Cadherin (24E10) Rabbit mAb (SignalStar™ Conjugate 0103)8490550 µlH MRabbit IgG
Complementary Oligo (CO-0103-647)2987022 µl

Storage

SignalStar conjugates are supplied in PBS (pH 7.2), less than 0.1% sodium azide, 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Complementary oligos are supplied in nuclease-free water. Store at -20°C. Do not aliquot the antibody. All components in this kit are stable for at least 12 months when stored at the recommended temperature.

Description

SignalStar multiplex immunohistochemistry (IHC) is an advanced technology for labeling multiple proteins simultaneously in tissue samples using specific primary antibodies and fluorescent detection reagents. This technology offers accuracy and reliability in visualizing and analyzing protein expression while maintaining spatial context and tissue architecture.

SignalStar Oligo-Antibody Pairs are compatible with the SignalStar Multiplex IHC Buffer Kits for use in fluorescent multiplex imaging experiments. This product includes the oligo-conjugated antibodies and complementary oligos required for labeling your target protein on up to 10 slides. SignalStar Multiplex IHC Buffer Kits are required to amplify and image the target signal. Multiple oligo-antibody pairs can be conveniently combined into a multiplex panel using the SignalStar Multiplex IHC Panel Builder. SignalStar Multiplex IHC Kits & Reagents are not compatible with all of Cell Signaling Technology® products and protocols that are recommended for use in immunohistochemical assays.

Specificity/Sensitivity

E-Cadherin (24E10) Rabbit mAb (SignalStar™ Conjugate 0103) detects endogenous levels of total E-cadherin protein. This antibody does not cross-react with related family members, such as N-cadherin.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro780 of human E-cadherin protein.

Background

Cadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellular domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development (1). The classic cadherin subfamily includes N-, P-, R-, B-, and E-cadherins, as well as about ten other members that are found in adherens junctions, a cellular structure near the apical surface of polarized epithelial cells. The cytoplasmic domain of classical cadherins interacts with β-catenin, γ-catenin (also called plakoglobin), and p120 catenin. β-catenin and γ-catenin associate with α-catenin, which links the cadherin-catenin complex to the actin cytoskeleton (1,2). While β- and γ-catenin play structural roles in the junctional complex, p120 regulates cadherin adhesive activity and trafficking (1-4). Investigators consider E-cadherin an active suppressor of invasion and growth of many epithelial cancers (1-3). Research studies indicate that cancer cells have upregulated N-cadherin in addition to loss of E-cadherin. This change in cadherin expression is called the "cadherin switch." N-cadherin cooperates with the FGF receptor, leading to overexpression of MMP-9 and cellular invasion (3). Research studies have shown that in endothelial cells, VE-cadherin signaling, expression, and localization correlate with vascular permeability and tumor angiogenesis (5,6). Investigators have also demonstrated that expression of P-cadherin, which is normally present in epithelial cells, is also altered in ovarian and other human cancers (7,8).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

SignalStar is a registered trademark of Cell Signaling Technology, Inc.

U.S. Patent No. 10,781,477, foreign equivalents, and child patents deriving therefrom.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human squamous cell lung carcinoma using MHC Class II (LGII-612.14) & CO-0105-488 SignalStar™ Oligo-Antibody Pair #55436 (green), E-Cadherin (24E10) & CO-0103-594 SignalStar™ Oligo-Antibody Pair #65400 (magenta), CD28 (D2Z4E) & CO-0081-647 SignalStar™ Oligo-Antibody Pair #96977 (red), α-Smooth Muscle Actin (D4K9N) & CO-0024-488 SignalStar™ Oligo-Antibody Pair #76133 (green), TCF1/TCF7 (C63D9) & CO-0006-594 SignalStar™ Oligo-Antibody Pair #34706 (yellow), and Arginase-1 (D4E3M) & CO-0075-647 SignalStar™ Oligo-Antibody Pair #44900 (white). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.
Immunohistochemistry Image 1: E-Cadherin (24E10) & CO-0103-647 SignalStar<sup>™</sup> Oligo-Antibody Pair
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human colorectal adenocarcinoma using E-Cadherin (24E10) & CO-0103-488 SignalStar™ Oligo-Antibody Pair #48503 (green). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.
Immunohistochemistry Image 2: E-Cadherin (24E10) & CO-0103-647 SignalStar<sup>™</sup> Oligo-Antibody Pair
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma using E-Cadherin (24E10) & CO-0103-594 SignalStar™ Oligo-Antibody Pair #65400 (yellow). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.
Immunohistochemistry Image 3: E-Cadherin (24E10) & CO-0103-647 SignalStar<sup>™</sup> Oligo-Antibody Pair
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using E-Cadherin (24E10) & CO-0103-647 SignalStar™ Oligo-Antibody Pair #31774 (red). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.
Immunohistochemistry Image 4: E-Cadherin (24E10) & CO-0103-647 SignalStar<sup>™</sup> Oligo-Antibody Pair
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human squamous cell lung carcinoma using E-Cadherin (24E10) & CO-0103-750 SignalStar™ Oligo-Antibody Pair #65506 (cyan). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.
Immunohistochemistry Image 5: E-Cadherin (24E10) & CO-0103-647 SignalStar<sup>™</sup> Oligo-Antibody Pair
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human squamous cell lung carcinoma using E-Cadherin (24E10) & CO-0103-750 SignalStar™ Oligo-Antibody Pair #65506 (left, cyan) and DAPI #4083 (left, blue) compared to chromogenic immunohistochemical analysis of a serial section of paraffin-embedded human squamous cell lung carcinoma using E-Cadherin (24E10) Rabbit mAb  #3195 (right). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.
Immunohistochemistry Image 6: E-Cadherin (24E10) & CO-0103-647 SignalStar<sup>™</sup> Oligo-Antibody Pair
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded mouse thymus using CD28 (D2Z4E) & CO-0081-488 SignalStar™ Oligo-Antibody Pair #52935 (green), and E-Cadherin (24E10) & CO-0103-647 SignalStar™ Oligo-Antibody Pair #31774 (red). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.
Immunohistochemistry Image 7: E-Cadherin (24E10) & CO-0103-647 SignalStar<sup>™</sup> Oligo-Antibody Pair
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human squamous cell lung carcinoma using MHC Class II (LGII-612.14) & CO-0105-488 SignalStar™ Oligo-Antibody Pair #55436 (green), E-Cadherin (24E10) & CO-0103-594 SignalStar™ Oligo-Antibody Pair #65400 (yellow), and CD28 (D2Z4E) & CO-0081-647 SignalStar™ Oligo-Antibody Pair #96977 (red). All fluorophores have been assigned a pseudocolor, as indicated. 
Immunohistochemistry Image 1: E-Cadherin (24E10) & CO-0103-647 SignalStar<sup>™</sup> Oligo-Antibody Pair
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.