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PhosphoSitePlus® Resource

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Western blot analysis of extracts from OVCAR8 cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® ABIN-1 siRNA I #6603 (+) or SignalSilence® ABIN-1 siRNA II (+), using ABIN-1 Antibody #4664 (upper) or β-Tubulin (9F3) Rabbit mAb #2128 (lower). The ABIN-1 Antibody confirms silencing of ABIN-1 expression, while the β-Tubulin (9F3) Rabbit mAb is used as a loading control.

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Product Usage Information

CST recommends transfection with 100 nM ABIN-1 siRNA II 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.

Each vial contains the equivalent of 100 transfections, which corresponds to a final siRNA concentration of 100 nM per transfection in a 24-well plate with a total volume of 300 μl per well.

Storage: SignalSilence® siRNA is supplied in RNAse-free water. Aliquot and store at -20ºC.

Product Description

SignalSilence® ABIN-1 siRNA II from Cell Signaling Technology (CST) allows the researcher to specifically inhibit ABIN-1 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

The ABIN family (ABIN-1, -2, and -3) is a group of adaptor proteins that associate and cooperate with A20/TNFAIP3 (1), a ubiquitin editing protein that inhibits the key inflammatory transcription factor NF-κB (2-4). Mechanistically, A20 acts by regulating the ubiquitination of the kinase RIP, which leads to inhibition of the IKK complex (5).

1.  Verstrepen, L. et al. (2009) Biochem Pharmacol 78, 105-14.

2.  Beyaert, R. et al. (2000) Biochem Pharmacol 60, 1143-51.

3.  Lee, E.G. et al. (2000) Science 289, 2350-4.

4.  Dixit, V.M. et al. (1990) J Biol Chem 265, 2973-8.

5.  Wertz, I.E. et al. (2004) Nature 430, 694-9.

Entrez-Gene Id 10318
Swiss-Prot Acc. Q15025

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SignalSilence is a registered trademark of Cell Signaling Technology, Inc.

SignalSilence® ABIN-1 siRNA II