CST recommends transfection with 100 nM Caspase-3 siRNA. Decreased Caspase-3 expression was observed 24-72 hours post-transfection. See Protocol for transfection procedure.
Each vial contains the equivalent of 100 transfections, which corresponds to a final siRNA concentration of 100 nM per transfection in a 24-well plate with a total volume of 300 μl per well.
SignalSilence® siRNA is supplied in RNAse-free water. Aliquot and store at -20ºC.
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