#P60520
11345
Product Information
Product Usage Information
CST recommends transfection with 100 nM SignalSilence® GABARAPL2 siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow the protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.
Each vial contains the equivalent of 100 transfections, which corresponds to a final siRNA concentration of 100 nM per transfection in a 24-well plate with a total volume of 300 μl per well.
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Product Description
Background
GABAA receptor associated protein (GABARAP) is an Atg8 family protein with a key role in autophagy, which was originally discovered as a protein associated with the GABAA receptor regulating receptor trafficking to the plasma membrane (1). Proteins in this family, including microtubule-associated protein light chain 3 (LC3) and GATE-16 (GABARAPL2), become incorporated into the autophagosomal membranes following autophagic stimuli such as starvation (2). Like the other family members, GABARAP is cleaved at its carboxyl terminus, which leads to conjugation by either of the phospholipids phosphatidylethanolamine or phosphatidylserine (3,4). This processing converts GABARAP from a type I to a type II membrane bound form involved in autophagosome biogenesis. Processing of GABARAP involves cleavage by Atg4 family members (5,6) followed by conjugation by the E1 and E2 like enzymes Atg7 and Atg3 (7,8). GABARAPL1/GEC1, a protein that is highly related to GABARAP, was identified as an estrogen inducible gene, and is also associated with autophagosomes (9-11).
- Wang, H. et al. (1999) Nature 397, 69-72.
- Shpilka, T. et al. (2011) Genome Biol 12, 226.
- Kabeya, Y. et al. (2004) J Cell Sci 117, 2805-12.
- Sou, Y.S. et al. (2006) J Biol Chem 281, 3017-24.
- Tanida, I. et al. (2004) J Biol Chem 279, 36268-76.
- Hemelaar, J. et al. (2003) J Biol Chem 278, 51841-50.
- Tanida, I. et al. (2001) J Biol Chem 276, 1701-6.
- Tanida, I. et al. (2002) J Biol Chem 277, 13739-44.
- Chakrama, F.Z. et al. (2010) Autophagy 6, 495-505.
- Pellerin, I. et al. (1993) Mol Cell Endocrinol 90, R17-21.
- Vernier-Magnin, S. et al. (2001) Biochem Biophys Res Commun 284, 118-25.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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