#O60313
4976
Product Information
Product Usage Information
CST recommends transfection with 100 nM SignalSilence® OPA1 siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.
Each vial contains the equivalent of 100 transfections, which corresponds to a final siRNA concentration of 100 nM per transfection in a 24-well plate with a total volume of 300 μl per well.
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Product Description
Background
Changes in mitochondrial dynamics regulated by environmental cues affect mitochondrial size and shape and have been shown to dramatically impact mitochondrial metabolism, apoptosis, and autophagy (1). These processes are largely controlled by mitochondrial dynamin-related GTPases, including mitofusin-1, mitofusin-2, OPA1, and DRP1. DRP1 regulates mitochondrial fission, while the mitofusins and OPA1 control fusion at the outer and inner mitochondrial membrane, respectively.
OPA1, or Optic Atrophy 1, was originally identified as a genetic cause for Autosomal Dominant Optic Atrophy, a neuropathy resulting in progressive visual loss (2,3). OPA1 is a widely expressed protein localized to the inner mitochondrial membrane, which regulates mitochondrial fusion and cristae morphology and protects against apoptosis (4-6). OPA1 activity is tightly regulated through alternative splicing and post-translational modifications including complex proteolytic processing by multiple proteases (7-12). In addition, OPA1 expression can be induced under conditions of metabolic demand through a pathway involving Parkin induced NF-κB activation (13).
- Kasahara, A. and Scorrano, L. (2014) Trends Cell Biol 24, 761-70.
- Delettre, C. et al. (2000) Nat Genet 26, 207-10.
- Alexander, C. et al. (2000) Nat Genet 26, 211-5.
- Frezza, C. et al. (2006) Cell 126, 177-89.
- Olichon, A. et al. (2003) J Biol Chem 278, 7743-6.
- Griparic, L. et al. (2004) J Biol Chem 279, 18792-8.
- Delettre, C. et al. (2001) Hum Genet 109, 584-91.
- Olichon, A. et al. (2007) Cell Death Differ 14, 682-92.
- Ishihara, N. et al. (2006) EMBO J 25, 2966-77.
- Cipolat, S. et al. (2006) Cell 126, 163-75.
- Griparic, L. et al. (2007) J Cell Biol 178, 757-64.
- Merkwirth, C. et al. (2008) Genes Dev 22, 476-88.
- Müller-Rischart, A.K. et al. (2013) Mol Cell 49, 908-21.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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