#O15350
7161
Product Information
Product Usage Information
CST recommends transfection with 50-100 nM p73 siRNA 48 hours prior to cell lysis. See protocol for transfection procedure.
Storage
Product Description
Background
The p53 family member, p73, exists in multiple isoforms/splice variants and can induce apoptosis and cell cycle arrest in response to DNA damage via its activity as a transcription regulator (1-3). Upon DNA damage, p73 is phosphorylated at Tyr99 by c-Abl, causing translocation to the nuclear matrix (4). DNA damage-induced acetylation of p73 at Lys321 by the acetyltransferase p300 has also been reported to enhance transcription of genes including that of p53AIP1 (5). Another report, however, indicates that p300 does not acetylate full-length p73 in vivo (6).
RNA interference has been used to block p73 expression in SW480 cells, resulting in chemoresistance (7).
- Kaghad, M. et al. (1997) Cell 90, 809-19.
- Jost, C.A. et al. (1997) Nature 389, 191-4.
- De Laurenzi, V.D. et al. (1999) Cell Death Differ 6, 389-90.
- Ben-Yehoyada, M. et al. (2003) J Biol Chem 278, 34475-82.
- Costanzo, A. et al. (2002) Mol Cell 9, 175-86.
- Zeng, X. et al. (2001) J Biol Chem 276, 48-52.
- Irwin, M.S. et al. (2003) Cancer Cell 3, 403-10.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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Limited Uses
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