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6411
SignalSilence® Pool Caspase 3 siRNA
siRNA

SignalSilence® Pool Caspase 3 siRNA #6411

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# Product Name Application Reactivity
H
H
Western Blotting

Western blot analysis of extracts from HeLa cells 48 hours following mock transfection, transfection with non-targeted (control) siRNA or transfection with Caspase-3 siRNA (33 nM). Caspase-3 was detected using Caspase-3 Rabbit Monoclonal Antibody #9665, and p42 MAPK was detected using p42 MAPK Antibody #9108 (left). The same lysates were also probed with antibodies against Caspase-7 #9492 (middle) or Caspase-8 #9746 (right) to confirm siRNA specificity.

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Fluorescent Detection

Fluorescent detection of SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 in living HeLa cells 24 hours post-transfection, demonstrating nearly 100% transfection efficiency.

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CST recommends transfection with 33 nM Caspase-3 siRNA. Decreased Caspase-3 expression was seen 24-72 hours post-transfection. See Protocol for transfection procedure.

Storage:

SignalSilence® siRNA is supplied in RNAse-free water. Aliquot and store at -20ºC.

SignalSilence® Pool Caspase-3 siRNA allows the researcher to specifically inhibit Caspase-3 expression using RNA interference, a method in which gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. The Caspase-3 siRNA is a hetergeneous mixture of 21-22 bp siRNAs. A 433 bp cDNA template of caspase-3 was transcribed by T7 RNA polymerase to create double-stranded RNA (dsRNA). RNase III was used to cleave the dsRNA in the presence of manganese buffer to 21-22 bp siRNA. All SignalSilence® siRNA are rigorously tested in-house and have been shown to reduce protein expression in specified cell lines.

Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires the aspartic acid residue at the P1 position (2).

  1. Fernandes-Alnemri, T. et al. (1994) J Biol Chem 269, 30761-4.
  2. Nicholson, D. W. et al. (1995) Nature 376, 37-43.
Entrez-Gene Id
836
Swiss-Prot Acc.
P42574
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SignalSilence is a registered trademark of Cell Signaling Technology, Inc.
Limited Use Label License, RNA interference: This product is licensed under European Patent 1144623 and foreign equivalents from Ribopharma AG, Kulmbach, Germany and is provided only for use in non-commercial research specifically excluding use (a) in drug discovery or drug development, including target identification or target validation, by or on behalf of a commercial entity, (b) for contract research or commercial screening services, (c) for the production or manufacture of siRNA-related products for sale, or (d) for the generation of commercial databases for sale to Third Parties. Information about licenses for these and other commercial uses is available from Ribopharma AG, Fritz-Hornschuch-Str. 9, D-95326 Kulmbach, Germany.

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