IP
H M R Mk
Endogenous
150
Rabbit IgG
#Q8N122
57521
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Immunoprecipitation | 1:20 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Raptor (24C12) Rabbit mAb is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human Raptor.
Product Description
| MW (kDa) | 150 |
Background
The regulatory associated protein of mTOR (Raptor) was identified as an mTOR binding partner that mediates mTOR signaling to downstream targets (1,2). Raptor binds to mTOR substrates, including 4E-BP1 and p70 S6 kinase, through their TOR signaling (TOS) motifs and is required for mTOR-mediated phosphorylation of these substrates (3,4). Binding of the FKBP12-rapamycin complex to mTOR inhibits the mTOR-raptor interaction, suggesting a mechanism for rapamycin's specific inhibition of mTOR signaling (5). This mTOR-raptor interaction and its regulation by nutrients and/or rapamycin is dependent on a protein called GβL (6). GβL is also part of the rapamycin-insensitive complex between mTOR and rictor (rapamycin-insensitive companion of mTOR), and may mediate rictor-mTOR signaling to downstream targets including PKCα (7). Furthermore, the rictor-mTOR complex has been identified as the previously elusive PDK2 responsible for the phosphorylation of Akt/PKB on Ser473, facilitating phosphorylation of Akt/PKB on Thr308 by PDK1 and required for the full activation of Akt/PKB (8).
Recently raptor has been identified as a direct substrate of the AMP-activated protein kinase (AMPK) (9). AMPK phosphorylates raptor on Ser722/Ser792 (9). This phosphorylation is essential for inhibition of the raptor-containing mTOR complex 1 (mTORC1) and induces cell cycle arrest when cells are stressed for energy (9). These findings suggest that raptor is a critical switch that correlates cell cycle progression with energy status.
- Hara, K. et al. (2002) Cell 110, 177-89.
- Kim, D. et al. (2002) Cell 110, 163-75.
- Beugnet, A. et al. (2003) J. Biol. Chem. 278, 40717-22.
- Nojima, H. et al. (2003) J. Biol. Chem. 278, 15461-64.
- Oshiro, N. et al. (2004) Genes Cells 9, 359-66.
- Kim, D. H. et al. (2003) Mol. Cell 11, 895-904.
- Sarbassov, D. et al. (2004) Curr. Biol. 14, 1296-302.
- Sarbassov, D.D. et al. (2005) Science 307, 1098-101.
- Gwinn, D.M. et al. (2008) Mol Cell 30, 214-26.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Applications Key
IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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