This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated Sepharose® beads. GLI1 (L42B10) Mouse mAb (Sepharose® Bead Conjugate) is useful for the immunoprecipitation of GLI1. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated GLI1 (L42B10) Mouse mAb #2643.
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol. Store at –20°C. Do not aliquot the antibodies.
GLI1 (L42B10) Mouse mAb (Sepharose® Bead Conjugate) recognizes endogenous levels of total GLI1 protein.
Monoclonal antibody is produced by immunizing animals with a recombinant human GLI1 protein.
GLI was first identified as a gene amplified in a malignant glioma (1) capable of transforming primary cells in cooperation with adenovirus E1A (2). GLI belongs to the Kruppel family of zinc finger proteins that includes three mammalian GLI proteins: GLI1, GLI2, and GLI3 (3). These GLI proteins are similar to the Drosophila homolog Cubitus interruptus (Ci) and function as transcription factors activated by the Hedgehog signaling pathway. Hedgehog signaling plays an important role in animal development, and research studies have shown that this pathway is aberrantly activated in many types of cancers (4,5).
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