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5873
Mouse IgG (Magnetic Bead Conjugate)
WB & IP Reagents

Mouse IgG (Magnetic Bead Conjugate) #5873

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  1. IP

Immunoprecipitation using NIH/3T3 (lane 1 and 2) and C6 (lane 3 and 4) cell extracts using Mouse IgG (Magnetic Bead Conjugate) (lane 1 and 3) or Akt (pan) (40D4) Mouse mAb (Magnetic Bead Conjugate) #5697 (lane 2 and 4). The western blot was probed using Akt (pan) (C67E7) Rabbit mAb #4691.

To Purchase # 5873S
Product # Size Price
5873S
400 µl $ 115

Supporting Data

Concentration 10 mg/ml
SENSITIVITY
Isotype Mouse 

Application Key:

  • W-Western
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Description

This Cell Signaling Technology normal mouse IgG (whole molecule) containing predominantly IgG1 and IgG2a isotypes and small amounts of IgG2b and IgG3 is immobilized by the covalent reaction of hydrazinonicotinamide-modifed antibody with formylbenzamide-modified magnetic beads. Mouse IgG (Magnetic Bead Conjugate) is useful to determine non-specific immunoprecipitation complexes.

Product Usage Information

Application Dilution
Immunoprecipitation 1:20

Storage:

Supplied in PBS Buffer (pH 7.2), 0.1% Tween® 20. Store at 4°C. Do not aliquot the antibodies.

Specificity / Sensitivity

Normal mouse IgG is not expected to recognize any specific antigen.

Source / Purification

Normal mouse IgG was isolated by affinity chromatography from naive mouse serum.

Background

Control antibodies are used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions.

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.