Immunoprecipitation using NIH/3T3 (lane 1 and 2) and C6 (lane 3 and 4) cell extracts using Mouse IgG (Magnetic Bead Conjugate) (lane 1 and 3) or Akt (pan) (40D4) Mouse mAb (Magnetic Bead Conjugate) #5697 (lane 2 and 4). The western blot was probed using Akt (pan) (C67E7) Rabbit mAb #4691.
This Cell Signaling Technology normal mouse IgG (whole molecule) containing predominantly IgG1 and IgG2a isotypes and small amounts of IgG2b and IgG3 is immobilized by the covalent reaction of hydrazinonicotinamide-modifed antibody with formylbenzamide-modified magnetic beads. Mouse IgG (Magnetic Bead Conjugate) is useful to determine non-specific immunoprecipitation complexes.
Supplied in PBS Buffer (pH 7.2), 0.1% Tween® 20. Store at 4°C. Do not aliquot the antibodies.
Normal mouse IgG is not expected to recognize any specific antigen.
Normal mouse IgG was isolated by affinity chromatography from naive mouse serum.
Control antibodies are used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.