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3420
Mouse IgG (Sepharose® Bead Conjugate)
WB & IP Reagents
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Mouse IgG (Sepharose® Bead Conjugate) #3420

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  1. IP

Western blot analysis of extracts from COS cells transfected with Myc-Chorionic Somatomammotropin Hormone-Like 1 (CSL) protein (lane 1). Immunoprecipitation of transfected COS lysates with Mouse IgG (Sepharose® Bead Conjugate) (lane 2) or Myc-Tag (9B11) (Sepharose® Bead Conjugate) Mouse mAb #3400 (lane 3). Blot was probed with Myc Tag (71D10) Rabbit mAb #2278.

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To Purchase # 3420S
Product # Size Price
3420S		 400 µl $ 115

Custom Formulation

Supporting Data

Concentration 5000 bead
SENSITIVITY
Isotype Mouse 

Application Key:

  • W-Western
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Description

This Cell Signaling Technology normal mouse IgG (whole molecule) containing predominantly IgG1 and IgG2a isotypes and small amounts of IgG2b and IgG3 is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated Sepharose® beads. Mouse IgG (Sepharose® Bead Conjugate) is useful to determine non-specific immunoprecipitation complexes.

Product Usage Information

Application Dilution
Immunoprecipitation 1:20

Storage:

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol. Store at –20°C. Do not aliquot the antibodies.

Specificity / Sensitivity

Normal mouse IgG is not expected to recognize any specific antigen.

Source / Purification

Normal mouse IgG was isolated by affinity chromatography from naive mouse serum.

Background

Control antibodies are used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions.

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.