This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated Sepharose® beads. Phospho-IκBα (Ser32/36) (5A5) Mouse mAb (Sepharose® Bead Conjugate) is useful for the immunoprecipitation of phosphorylated IκBα.
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol. Store at –20°C. Do not aliquot the antibodies.
Phospho-IκBα (Ser32/36) (5A5) Mouse mAb (Sepharose® Bead Conjugate) detects endogenous levels of IκBα only when phosphorylated at Ser32/36.Species predicted to react based on 100% sequence homology:
Bovine, Dog, Pig, Guinea Pig
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser32/36 of human IκBα.
The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins (1-3). Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB (3-7). IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified (8).
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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.